MASS SPECTROMETRY OF INVOLATILE BIOMOLECULES

质谱学是分析不可缺少的技术。 具有重要生物学意义的化合物。因此，发展 大的、不稳定的、 极性分子对科学研究的进展具有重要意义。 生物医学、生化和生物科学。 我们的总目标是扩大有机物质的用途 生化、生物医学和生物科学中的光谱学 促垂体激素的一般和内分泌学研究 尤其是荷尔蒙。我们的具体目标如下：i) 研究支配环境变化的物理和化学过程 从液体和固体基质中喷出二次离子 受到能量在以下范围的粒子的轰击 5-50keV，以及ii)发展一种或多种方法，能够 检测小于或等于1pmole的单个寡肽 而不会不适当地牺牲质量的特殊性 光谱分析。 当样品被提取时，质谱仪的用处就会降低。 由于质量范围、灵敏度或 复合类型。当前的质谱学灵敏度为 在研究方面尤其受限，例如关于激素或 疾病的分子标记，其中分析物的可用性 受到活体采样要求的严重限制。 在过去十年中发现的电离技术 极大地增加了分子的种类和尺寸 质谱分析；然而，已经注意到， 在最高、可电离分子的灵敏度方面有类似的提高 在装满之前需要重量(10,000-20,000道尔顿) 可以利用这些新的离子源。达到的程度 哪种敏感度可以在不过度牺牲的情况下得到提高 定性特异性是基础研究和应用研究的问题。 本提案中描述的调查方案不会 仅对我们的理解做出总体贡献 解吸电离过程，但它将利用这一宝贵的 使质量显著减少的知识体系 促垂体激素的光谱检出限 这一成就将显著扩大……的视野 内分泌学研究。

Mass spectrometry is an indispensable technique for the analysis of biologically important compounds. Thus, the development of techniques for mass spectrometric determinations of large, labile, polar molecules is of great importance to progress in the biomedical, biochemical, and biological sciences. Our general objective is to expand the utility of organic mass spectrometry in biochemical, biomedical, and biological sciences generally and in endocrinological research on hypophysiotropic hormones particularly. Our specific aims are as follows: i) to investigate the physical and chemical processes that govern the ejection of secondary ions from liquid and solid matrices that are subjected to bomdardment by particles with energies ranging from 5-50 keV, and ii) to evolve one or more methods capable of detecting less than or equal to 1 pmole of individual oligopeptides without unduly sacrificing the qualitative specificity of the mass spectrometry. The usefulness of mass spectrometry is reduced when samples cannot be analyzed due to bounds on mass range, sensitivity, or compound types. Current mass spectrometric sensitivities are particularly restrictive in research, such as that on hormones or molecular markers for diseases, where the availability of analyte is severely limited by requirements for sampling in vivo. Ionization techniques discovered during the last decade have greatly increased the variety and size of molecules amenable to mass spectrometric analysis; it has been noted, however, that comparable gains in sensitivity at the highest, ionizable molecular weights (10,000-20,000 daltons) will be required before full advantage can be taken of these new ion sources. The extent to which sensitivity can be increased without unduly sacrificing qualitative specificity is a matter for basic and applied research. The program of investigation described in this proposal would not only make general contributions to our understanding of desorption ionization processes, but it would utilize that valuable body of knowledge to bring about a significant reduction in mass spectrometric detection limits for hypophysiotropic hormones, an accomplishment that would markedly expand the horizons of research in endocrinology.