MASS SPECTROMETRY OF INVOLATILE BIOMOLECULES

质谱学是分析化学物质不可缺少的技术 具有重要生物学意义的化合物。因此，技术的发展 对大的、不稳定的、极性分子的质谱分析是非常有意义的 在生物医学、生物化学和生物学方面取得进展的重要性 科学。我们的总目标是扩大有机食品的用途 生物医学和生物化学中的质谱学。 我们的具体目标如下：1.通过实验研究 支配二次离子抛射的物理和化学过程 KEV粒子轰击过程中的固体和液体基质； 阐述和测试沉积和分配的机械假说 将能量转化为有机样品基质，用于碎裂和电离 关于溅射的分子；3.利用基本过程的知识 优化二次离子源设计和实验方案 大分子、不稳定、极性分子的质谱学研究 生物化学和生物兴趣。液态金属离子一次源 是实现我们实验目标的关键。我们已经率先使用了 这种独特而多功能的离子源在二次离子质谱仪中的应用 (模拟人生)。 当重要的生物分子出现时，质谱学的作用就会降低。 由于质量范围、灵敏度或 复合类型。最近，使用原子离子或 中性原子主光束和液体样品矩阵做出了贡献 重要的是消除了对质谱学的大量限制 分析热不稳定、高相对分子质量的有机化合物。它 我们的前提是导致能力增强的研究 将在应用质量方面做出重大贡献 光谱分析在生物医学和生物化学中的重要问题。这, 然后，是我们的具体目标与我们长期搁浅的将军之间的关系 目标。

Mass spectrometry is an indispensable technique for the analysis of biologically important compounds. Thus, the development of techniques for mass spectrometric analysis of large, labile, polar molecules is of great importance to progress in the biomedical, biochemical, and biological sciences. It is our general objective to extend the utility of organic mass spectrometry in biomedicine and biochemistry. Our specific aims are as follow: 1. experimentally investigate the physical and chemical processes which govern the ejection of secondary ions from solid and liquid matrices during bombardment with keV particles; 2. formulate and test mechanistic hypotheses for deposition and partitioning of energy into organic sample matrices and for fragmentation and ionization of sputtered molecules; 3. utilize knowledge of the fundamental processes to optimize source designs and experimental protocols for secondary ion mass spectrometry of high molecular weight, labile, polar molecules of biochemical and biological interest. The liquid metal ion primary source is the key to achieving our experimental aims. We have pioneered the use of this unique and versatile ion source in secondary ion mass spectrometry (SIMS). The usefulness of mass spectrometry is reduced when important biomolecules cannot be analyzed due to limitations on mass range, sensitivity or compound types. Relatively recently, SIMS employing either atomic ion or neutral atom primary beams and liquid sample matrices has contributed significantly to removing numerous restrictions on the mass spectrometric analysis of thermally labile, high molecular weight, organic compounds. It is our premise that research which leads to enhancement of the capabilities of molecular SIMS will have made a major contribution toward applying mass spectrometry to important problems in biomedicine and biochemistry. This, then, is how our specific aims are related to our long-stranding general objective.