A Research Grade MALDI TOF Mass Spectrometer

研究级 MALDI TOF 质谱仪

• 批准号: 6440904
• 负责人: DOUGLAS F BAROFSKY
• 金额: $ 26.04万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440904
• 关键词: biomedical equipment purchase; mass spectrometry; matrix assisted laser desorption ionization

The Department of Chemistry and the Environmental Health Sciences Center (EHSC) at Oregon State University (OSU) request funds to purchase a new high performance MALDI time-of-flight mass spectrometer system. This instrument is needed to accommodate the rapidly growing demand at OSU and the Oregon research community at large for a range of MALDI mass spectrometric analyses. The existing MALDI instrumentation in OSU's Mass Spectrometry Facility does not have the sensitivity, resolving power, mass accuracy, and sample throughput required to support this demand. In particular, the existing instruments are not capable of providing either the quality or quantity of information sought by the investigators designated as major users in this proposal. The new instrument would be used to produce mass- maps of peptides produced by enzymatic and chemical digestion of proteins in efforts to identify the proteins, to obtain sequence tags to aid in the identification of proteins and of sites of phosphorylation or other such modifications within the proteins, to monitor the exchange of hydrogen and deuterium in studies on folding intermediates and conformational states resulting from modifications and ligand interactions, and to monitor the spontaneous deamination of cytosine in double-stranded DNA due to conformational effects. All of these applications would substantially benefit research supported by NIH. Finally, the new mass spectrometer would support a large number of minor users both on- and off-campus who are among those seeking high performance MALDI analysis in their research.

俄勒冈州州立大学（OSU）化学系和环境健康科学中心（EHSC）申请资金购买一种新的高性能MALDI飞行时间质谱仪系统。这种仪器是需要适应在俄勒冈州立大学和俄勒冈州的研究社区在广泛的MALDI质谱分析范围的快速增长的需求。OSU质谱设施中现有的MALDI仪器不具备支持这一需求所需的灵敏度、分辨能力、质量准确度和样品通量。特别是，现有文书无法提供本提案中指定为主要用户的调查人员所要求的信息的质量或数量。新仪器将用于产生通过蛋白质的酶促和化学消化产生的肽的质谱图，以鉴定蛋白质，获得序列标签以帮助鉴定蛋白质和蛋白质内的磷酸化位点或其他此类修饰，在研究折叠中间体和由修饰和配体引起的构象状态时监测氢和氘的交换的相互作用，并监测自发脱氨基的胞嘧啶在双链DNA由于构象效应。所有这些应用都将大大有利于NIH支持的研究。最后，新的质谱仪将支持大量的校园内外的小用户，他们在研究中寻求高性能的MALDI分析。