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Host-pathogen interactions important in the movement of Campylobacter jejuni from the broiler chicken gut to edible tissues (CampAttack)

宿主与病原体的相互作用对于空肠弯曲杆菌从肉鸡肠道向可食用组织的移动很重要（CampAttack）

基本信息

批准号：
BB/R019142/1
负责人：
Thomas Stephens Wilkinson
金额：
$ 48.2万
依托单位：
Swansea University
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FR019142%2F1
关键词：
Host pathogen interactions important movement

项目摘要

Sustainable production of safe chicken is an international priority and it is estimated that in the next 20 years chicken production will have to quadruple to satisfy growing global demand. The key question is whether this can be done in a way that does not increase the public health threat of contaminated chicken meat and preserves chicken health and welfare. Most chicken meat consumed internationally is produced in large-scale intensive (broiler) systems and most birds in the UK (>75%) are Campylobacter-positive at retail, mainly with C. jejuni, posing a huge public health threat. Campylobacter is the most common cause of bacterial diarrhoea in the UK and despite millions of pounds of research funding it is estimated that contaminated chicken caused >500000 human campylobacteriosis cases in the UK in 2016 with around 100 deaths, mainly in elderly people. Infection is characterised by severe abdominal pain and acute (sometimes bloody) diarrhoea and costs the UK economy over £1 billion per year. In addition, Campylobacter are not only major chicken-associated human pathogens, they also compromise the health, welfare and performance of broilers. Campylobacter contamination of chicken takes two forms. First, surface contamination of carcasses, as a result of spillage of gut contents during slaughter, can lead to cross-contamination in the kitchen. Second, and perhaps of greater importance than currently thought, is contamination within muscle and liver tissues, which increases the health risk by facilitating bacterial survival during cooking. Until recently it was believed that Campylobacter only colonised the lower gut of the chicken (the caecum). However, spread from the gut to edible tissues is associated with the ability of certain Campylobacter strains to colonise the upper intestine of the chicken, where the gut lining (mucosa) is more easily damaged. As Campylobacter comprise a diverse population in broilers, with different strains varying in their effects on gut integrity and their ability to spread to edible tissues like liver and muscle, it is important to better understand the host-pathogen interactions of different types if the bacteria are to be controlled in chickens and the public health threat reduced. In particular, it is essential to identify the key host immune responses and the bacterial genes most important in these interactions - and in colonisation of the whole gut and extra-intestinal spread. This information, which is currently not available, is essential for the development of immunity-based and other control measures.This multidisciplinary research programme will enhance understanding of the influence of Campylobacter strain on bird gut health, host innate immune responses and spread to edible tissues and thus the public health threat. The quantitative information and modelling will be used to give direct advice to industry about Campylobacter infection biology in broiler chickens, providing an unprecedented basis for interventions to mitigate the on-going challenge of Campylobacter contamination in chicken meat. These interventions potentially include new vaccines and/or genetically more resistant chickens.

安全鸡肉的可持续生产是一个国际优先事项，据估计，在今后20年内，鸡肉产量必须翻两番，才能满足日益增长的全球需求。关键问题是，这是否可以在不增加受污染鸡肉的公共卫生威胁的情况下进行，并保护鸡的健康和福利。国际上消费的大多数鸡肉都是在大规模集约化（肉鸡）系统中生产的，英国的大多数家禽（>75%）在零售时均呈弯曲杆菌阳性，主要是C。jejuni，构成巨大的公共卫生威胁。弯曲杆菌是英国细菌性腹泻最常见的原因，尽管有数百万英镑的研究资金，但据估计，受污染的鸡肉在2016年在英国造成了超过50万例人类弯曲杆菌病，约有100人死亡，主要是老年人。感染的特征是严重的腹痛和急性（有时是血性）腹泻，每年给英国经济造成超过10亿英镑的损失。此外，弯曲杆菌不仅是与鸡相关的主要人类病原体，它们还损害肉鸡的健康、福利和生产性能。弯曲杆菌对鸡肉的污染有两种形式。首先，屠宰过程中内脏内容物溢出造成的屠体表面污染可能导致厨房交叉污染。其次，也许比目前认为的更重要的是肌肉和肝脏组织内的污染，这通过促进烹饪过程中细菌的存活而增加了健康风险。直到最近，人们才相信弯曲杆菌只在鸡的下消化道（盲肠）中定植。然而，从肠道到可食用组织的传播与某些弯曲杆菌菌株定殖鸡的上肠的能力有关，在上肠中，肠道衬里（粘膜）更容易受损。由于弯曲杆菌在肉鸡中包含不同的种群，不同菌株对肠道完整性的影响以及传播到肝脏和肌肉等可食用组织的能力各不相同，因此如果要在鸡中控制细菌并减少公共卫生威胁，那么更好地了解不同类型的宿主-病原体相互作用是很重要的。特别是，必须确定关键的宿主免疫反应和在这些相互作用中最重要的细菌基因-以及在整个肠道和肠外传播的定植中。这一多学科研究计划将加强了解弯曲杆菌菌株对鸟类肠道健康、宿主先天免疫反应和传播到可食用组织的影响，从而对公共卫生构成威胁。定量信息和建模将用于向行业提供有关肉鸡弯曲杆菌感染生物学的直接建议，为减轻鸡肉中弯曲杆菌污染的持续挑战的干预措施提供前所未有的基础。这些干预措施可能包括新疫苗和/或遗传上更具抗性的鸡。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Genome evolution and the emergence of pathogenicity in avian Escherichia coli.

基因组进化和致病性的出现在鸟类大肠杆菌中。

DOI：
10.1038/s41467-021-20988-w
发表时间：
2021-02-03
期刊：
Nature communications
影响因子：
16.6
作者：
Mageiros L;Méric G;Bayliss SC;Pensar J;Pascoe B;Mourkas E;Calland JK;Yahara K;Murray S;Wilkinson TS;Williams LK;Hitchings MD;Porter J;Kemmett K;Feil EJ;Jolley KA;Williams NJ;Corander J;Sheppard SK
通讯作者：
Sheppard SK

Coinfection Mechanisms ofCampylobacter andEscherichia coli in Human and Chicken Epithelial cells

人和鸡上皮细胞中弯曲杆菌和大肠杆菌的共感染机制