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Broiler gut health and C. jejuni infection: impacts of harvest management

肉鸡肠道健康和空肠弯曲菌感染：收获管理的影响

基本信息

批准号：
BB/M009610/1
负责人：
Thomas Stephens Wilkinson
金额：
$ 37.28万
依托单位：
Swansea University
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2015
资助国家：
英国
起止时间：
2015 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FM009610%2F1
关键词：
Broiler gut health C.jejuni

项目摘要

Life-End summarySustainable production of safe chicken is an international priority and preserving bird welfare is a key component of this. Current intensive (broiler) production can compromise bird health and welfare and food safety and there are strong links between poor bird welfare and the Campylobacter public health threat. Campylobacter is the most common cause of bacterial diarrhoea in the EU and despite millions of pounds of research funding it is estimated that contaminated chicken caused ~700000 human campylobacteriosis cases in the UK in 2013 with around 100 deaths. Infection is characterised by severe abdominal pain and acute (sometimes bloody) diarrhoea and costs the UK an estimated £1 billion per year. Campylobacter contamination of chicken takes two forms. First, surface contamination of carcasses leads to cross-contamination in the kitchen. Second, and perhaps of greater importance than currently thought, contamination within muscle and liver tissues, increasing the health risk by facilitating bacterial survival during cooking.Chickens in poor production environments or exposed to stress are more susceptible to Campylobacter and in such birds the bacteria show greater extra-intestinal spread to edible tissues, possibly as a consequence of disturbance to the gut environment. Therefore, improvements in broiler welfare have great potential to improve public health but there is an urgent need for information on the effect of stress to inform targeted interventions to reduce Campylobacter in broiler chickens.One acutely stressful event in the life of broilers, in any production stream, is harvest when birds are removed from the farm for slaughter. We define the process as comprising: food withdrawal, catching, transport and stunning by either gas or electricity. Although there is a growing body of evidence that these stressors can increase Campylobacter growth rates as well as extra-intestinal spread, there is a paucity of data on their relative importance or how they may select for particular types of Campylobacter. By examining the harvest processes using large scale industry-relevant experimental conditions, state-of-the-art genomics, molecular microbiology and mathematical modelling techniques, we will determine the impact of harvest on gut health in broilers. We will combine this with a study to identify bacterial genetic determinants involved in extra-intestinal spread of Campylobacter to edible tissues. We will quantify the relative impact of each stage of harvest on the gut bacterial population and the physiology and immunity of the birds, and investigate the role these play in controlling extra-intestinal spread of Campylobacter. This multidisciplinary research programme will enhance understanding of the influence of the harvest process on bird gut health and Campylobacter. The quantitative information and modelling will be used to provide direct advice to industry about the elements of the harvest processes that provide the best opportunity for interventions that will mitigate the ongoing challenge of Campylobacter contamination in chicken meat.

安全鸡肉的可持续生产是国际优先事项，保护鸟类福利是其中的关键组成部分。目前的集约化（肉鸡）生产可能会损害鸟类的健康和福利以及食品安全，鸟类福利差与弯曲杆菌对公共卫生的威胁之间存在密切联系。弯曲杆菌是欧盟细菌性腹泻最常见的原因，尽管有数百万英镑的研究资金，但据估计，2013年受污染的鸡肉在英国造成了约70万例人类弯曲杆菌病，约100人死亡。感染的特征是剧烈腹痛和急性（有时是带血）腹泻，英国每年因感染造成的损失估计为10亿英镑。弯曲杆菌对鸡肉的污染有两种形式。首先，屠体的表面污染导致厨房的交叉污染。第二，也许比目前认为的更重要的是，肌肉和肝脏组织内的污染，通过在烹饪过程中促进细菌存活而增加健康风险。在恶劣的生产环境中或暴露于压力下的鸡更容易受到弯曲杆菌的影响，在这些鸡中，细菌显示出更大的肠道外传播到可食用组织，可能是肠道环境受到干扰的结果。因此，改善肉鸡福利有很大的潜力，以改善公众健康，但有迫切需要的信息的压力的影响，以告知有针对性的干预措施，以减少弯曲杆菌在肉鸡。一个急性应激事件在肉鸡的生活中，在任何生产流程，是收获时，鸟类从农场屠宰。我们将这个过程定义为：食物提取，捕捉，运输和用气体或电力击昏。虽然有越来越多的证据表明，这些压力因素可以增加弯曲杆菌的生长速度以及肠外传播，但关于它们的相对重要性或它们如何选择特定类型的弯曲杆菌的数据很少。通过使用大规模行业相关实验条件，最先进的基因组学，分子微生物学和数学建模技术检查收获过程，我们将确定收获对肉鸡肠道健康的影响。我们将联合收割机与一项研究相结合，以确定弯曲杆菌肠外传播到可食用组织的细菌遗传决定因素。我们将量化每个收获阶段对肠道细菌种群以及鸟类生理和免疫的相对影响，并研究这些在控制弯曲杆菌肠外传播中的作用。这一多学科研究计划将提高对家禽肠道健康和弯曲杆菌的收获过程的影响的理解。定量信息和建模将用于向行业提供有关收获过程要素的直接建议，这些要素为减轻鸡肉中弯曲杆菌污染的持续挑战提供了最佳干预机会。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Coinfection Mechanisms ofCampylobacter andEscherichia coli in Human and Chicken Epithelial cells

人和鸡上皮细胞中弯曲杆菌和大肠杆菌的共感染机制

DOI：
10.1099/acmi.fis2019.po0165
发表时间：
2020
期刊：
Access Microbiology
影响因子：
0
作者：
Hanford T
通讯作者：
Hanford T

Distribution and Genetic Profiles of Campylobacter in Commercial Broiler Production from Breeder to Slaughter in Thailand.

DOI：
10.1371/journal.pone.0149585
发表时间：
2016
期刊：
PloS one
影响因子：
3.7
作者：
Prachantasena S;Charununtakorn P;Muangnoicharoen S;Hankla L;Techawal N;Chaveerach P;Tuitemwong P;Chokesajjawatee N;Williams N;Humphrey T;Luangtongkum T
通讯作者：
Luangtongkum T

Reliably colonising broiler chickens with Campylobacter spp. using a litter-based method.

用弯曲杆菌可靠地定植肉鸡。

DOI：
10.1080/00071668.2018.1523538
发表时间：
2018
期刊：
British poultry science
影响因子：
2
作者：
Sandilands V
通讯作者：
Sandilands V

Domestication of Campylobacter jejuni NCTC 11168

空肠弯曲菌的驯化 NCTC 11168

DOI：
10.1101/591701
发表时间：
2019
期刊：
影响因子：
0
作者：
Pascoe B
通讯作者：
Pascoe B

Genome evolution and the emergence of pathogenicity in avian Escherichia coli.

基因组进化和致病性的出现在鸟类大肠杆菌中。

DOI：
10.1038/s41467-021-20988-w
发表时间：
2021-02-03
期刊：
Nature communications
影响因子：
16.6
作者：
Mageiros L;Méric G;Bayliss SC;Pensar J;Pascoe B;Mourkas E;Calland JK;Yahara K;Murray S;Wilkinson TS;Williams LK;Hitchings MD;Porter J;Kemmett K;Feil EJ;Jolley KA;Williams NJ;Corander J;Sheppard SK
通讯作者：
Sheppard SK