New Technology for High-Performance and High-Capacity Bio-separations: Protein Purification using "Hydrophobic Water" Solvent Systems

高性能和高容量生物分离新技术：使用“疏水水”溶剂系统进行蛋白质纯化

• 批准号: BB/R022364/1
• 负责人: Peter Nockemann
• 金额: $ 19.11万
• 依托单位: Queen's University Belfast
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR022364%2F1
• 关键词: New; Technology; Performance; Capacity; Bio

New Technology for High-Performance and High-Capacity Bio-separations: Protein Purification using "Hydrophobic Water" Solvent Systems in Countercurrent Chromatography ApparatusSummaryIn the biotechnology industries, the manufacture of high-value proteins, peptides and biomolecules require fast, low cost, reliable, GMP-compliant and scalable production methodologies. One scientific area used to achieve this, is the use of preparatory chromatography and related extraction technologies. It is proposed to use a combination of three relatively new technologies, namely: (1) ionic liquids, (2) modern high performance countercurrent chromatography (HPCCC) and centrifugal partition chromatography (CPC) and (3) co-solvent free, high water content microemulsions derived from catanionic surfactants. The microemulsion solvent systems proposed are a class of surface active, hydrophobic ionic liquids, which enable a new range of high performance, high capacity and environmentally friendly separations of peptides, proteins and other biomolecules. The mobile or carrier phase in these separations is usually water, which makes the process cheap and environmentally benign. This is a highly multidisciplinary project, which will combine elements of bioscience, biochemistry, chemical engineering, physical, and synthetic chemistry. This project will make use of ionic liquids solvent systems and microemulsion solvent systems in the solvent extraction and countercurrent chromatographic preparation, purification and isolation of peptides and proteins of commercial importance. This will initially use model separations of proteins, to develop and integrate the different technological parts of this new methodology, and then use this information to carry out separations of proteins mixtures from natural sources. Also, this project aims to scale up the protein separations by the use of high capacity CPC equipment installed in the QUILL Research Centre and employ automation to reduce the running costs of the separations. The ultimate goal of this project is to produce a general purpose, clean, water based, efficient, cost effective peptide and protein separation system, that can be exploited by the UK biotechnology industry and help in the growth of these sectors in the UK economy.

用于高性能和高容量生物分离的新技术：在逆流色谱装置中使用“疏水水”溶剂系统的蛋白质纯化概述在生物技术工业中，高价值蛋白质、肽和生物分子的制造需要快速、低成本、可靠、符合GMP和可扩展的生产方法。用于实现这一目标的一个科学领域是使用制备色谱法和相关提取技术。建议使用三种相对较新的技术的组合，即：（1）离子液体，（2）现代高效逆流色谱（HPCCC）和离心分配色谱（CPC）和（3）无共溶剂，高水含量的微乳液衍生自阴阳离子表面活性剂。所提出的微乳液溶剂系统是一类具有表面活性的疏水离子液体，其能够实现肽、蛋白质和其他生物分子的一系列新的高性能、高容量和环境友好的分离。这些分离中的移动的或载体相通常是水，这使得该方法便宜且环境友好。这是一个高度多学科的项目，它将结合生物科学，生物化学，化学工程，物理和合成化学的联合收割机元素。该项目将利用离子液体溶剂系统和微乳液溶剂系统进行溶剂萃取和逆流色谱制备，纯化和分离具有商业重要性的肽和蛋白质。这将首先使用蛋白质的模型分离，开发和整合这种新方法的不同技术部分，然后使用这些信息从天然来源中分离蛋白质混合物。此外，该项目旨在通过使用安装在QUILL研究中心的高容量CPC设备来扩大蛋白质分离，并采用自动化来降低分离的运行成本。该项目的最终目标是生产一种通用的、清洁的、基于水的、高效的、具有成本效益的肽和蛋白质分离系统，该系统可以被英国生物技术行业利用，并有助于英国经济中这些行业的增长。