Lipid-protein interplay in the mechanism of the sodium pump

钠泵机制中的脂质-蛋白质相互作用

• 批准号: DP150101112
• 负责人: A/Prof Ronald Clarke
• 金额: $ 23.48万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP150101112
• 关键词: Lipid; protein; interplay; mechanism; sodium

The sodium pump is the major energy-consuming enzyme of animal cells. Its ion pumping is essential to numerous physiological processes (e.g. nerve, muscle and kidney activity and the maintenance of cell volume). Because of its importance in so many cell functions, the enzyme must be able to respond to cellular conditions. Using measurements of the enzyme's activity in isolated membrane fragments and comparison with its behaviour in living cells, this project aims to determine how sodium pump activity is modulated by transmembrane electric potential and intramembrane electric field strength. Our project could provide fundamental new knowledge on how membrane protein function in general can be controlled by electrical properties of their lipid surroundings.

钠泵是动物细胞的主要耗能酶。它的离子泵对于许多生理过程（例如神经，肌肉和肾脏活动以及细胞体积的维持）至关重要。由于它在许多细胞功能中的重要性，这种酶必须能够对细胞条件做出反应。通过测量分离的膜片段中酶的活性并与其在活细胞中的行为进行比较，该项目旨在确定跨膜电位和膜内电场强度如何调节钠泵活性。我们的项目可以提供关于膜蛋白功能如何通过其脂质环境的电特性来控制的基本新知识。