In situ measurements of the electrostatic properties inside photosynthetic reaction centres: correlation with the energy conversion function of the protein

光合反应中心内静电特性的原位测量：与蛋白质能量转换功能的相关性

• 批准号: LX0454408
• 负责人: A/Prof Ronald Clarke
• 金额: $ 1.01万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Linkage - International
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LX0454408
• 关键词: situ; measurements; electrostatic; properties; inside

The photochemical reaction centre is a key protein complex involved in energy conversion. It converts solar energy into chemical energy as a transmembrane charge separation. Coupling of electron and proton transfer is catalysed at the level of a ubiquinone cofactor. In order to understand how the redox properties of this cofactor are fine tuned by the protein environment, we plan to probe the ubiquinone site using a voltage-sensitive fluorescent dye. This exciting multidisciplinary project will contribute to the understanding of how protein matrices influence and govern the midpoint redox potential of their cofactors and the environments of their

光化学反应中心是参与能量转换的关键蛋白质复合物。它通过跨膜电荷分离将太阳能转化为化学能。电子和质子转移的偶联在泛醌辅因子的水平上被催化。为了了解这种辅因子的氧化还原特性是如何通过蛋白质环境进行微调的，我们计划使用电压敏感的荧光染料来探测泛醌位点。这个令人兴奋的多学科项目将有助于了解蛋白质基质如何影响和管理其辅因子的中点氧化还原电位及其环境。