DESENSITIZATION OF CALCIOTROPIC HORMONE RECEPTORS

这项研究计划的长期目标是阐明 降钙素(CT)调节自身反应的机制 骨骼和肾脏等组织。有证据表明 激动剂中CT脱敏对腺苷酸环化酶(AC)的刺激 行动。尽管还有其他脱敏机制可以 发生时，导致脱敏的主要修饰部位是 感受器本身。与其他鸟嘌呤核苷酸结合调节剂(G) 蛋白偶联受体(GPCRs)，脱敏已被发现 由受体G蛋白解偶联、受体内化和 受体下调。我们将检验以下假设 关于CT反应脱敏的机制。 推测的羧基末端和第三个氨基酸残基 CT受体介导受体-G蛋白的胞内环 解偶联、受体内化和受体下调，其中 部分是通过刺激受体降解而发生的。为了测试这一点 假设，我们将追求以下具体目标：1)设计和 构建CT受体的合成基因。这个目标在什么时候 这一成果将对这一领域的研究产生重大的实际影响 因为它将极大地促进对这种受体的研究 分子遗传学技术。2)建立蜂窝系统 具有刺激和脱敏作用的CT受体cDNAs AC和磷脂酰肌醇特异性磷脂酶C(PPlase)， 受体内化和受体下调。3)确定 CT受体与G蛋白相互作用的结构特征 激活AC和PPlase的蛋白质(S)。4)确定结构 介导受体-G蛋白解偶联的CT受体的特征 受体内化和下调。分子遗传学 将被用来构建合成基因和突变受体 DNA。脱敏(或快速反应)，这限制了大小和 反应持续时间，限制药物的疗效 这些受体。因此，理解这个问题的一个重要原因 脱敏的机制是能够设计非肽类药物， 它不需要非肠道或鼻腔给药， 可以避免脱敏。这些药物可以提高疗效。 受体激动剂通过抑制脱敏或BE激动剂 他们自己的CT-R。这些药物可以用来治疗 骨质疏松。

The long-term objectives of this research program are to elucidate the mechanisms through which calcitonin (CT) regulates its own responses in tissues such as bone and kidney. Evidence has been presented that stimulation of adenylyl cyclase (AC) by CT desensitizes during agonist action. Although there are other mechanisms by which desensitization can occur, a major site of modification causing desensitization is the receptor itself. With other guanine nucleotide-binding regulatory (G) protein-coupled receptors (GPCRs), desensitization has been found to be caused by receptor-G protein uncoupling, receptor internalization and receptor down-regulation. We will test the following hypothesis regarding the mechanisms involved in desensitization of responses to CT. Amino acid residues in the putative carboxyl termini and third intracellular loops of the CT receptor mediate receptor-G protein uncoupling, receptor internalization and receptor down-regulation, which occurs in part by stimulating receptor degradation. To test this hypothesis, we will pursue the following Specific Aims: 1) To design and construct a synthetic gene for the CT receptor. This goal when accomplished will have a major practical impact on research in this field because it will greatly facilitate the study of this receptor by molecular genetic techniques. 2) To establish cell systems with transfected CT receptor cDNAs that show stimulation and desensitization of both AC and phosphoinoitide-specific phospholipase C (PPlase), receptor internalization and receptor down-regulation. 3) To determine the structural features of the CT receptor that interact with a G protein(s) to activate AC and PPlase. 4) To determine the structural features of the CT receptor that mediate receptor-G protein uncoupling, and receptor internalization and down-regulation. Molecular genetics will be used to construct the synthetic genes and to mutate the receptor DNAs. Desensitization (or tachyphylaxis), which limits the magnitude and duration of responses, limits the efficacy of drugs that act through these receptors. Therefore, one important reason to understand the mechanism of desensitization is to be able to design non-peptide drugs, which would not require parenteral nor intranasal administration, that may circumvent desensitization. These drugs could enhance the efficacy of receptor agonists by inhibiting desensitization or be agonists themselves of the CT-R. These agents could be used to treat osteoporosis.