BIOLOGY OF HHV8/KSHV G PROTEIN COUPLED RECEPTOR

HHV8/KSHV G 蛋白偶联受体的生物学

• 批准号: 6164248
• 负责人: MARVIN C GERSHENGORN
• 金额: $ 33.49万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6164248
• 关键词: G protein; Kaposi's sarcoma; biological signal transduction; human herpesvirus 8; interleukin 8; molecular cloning; molecular pathology; peptide analog; receptor binding; receptor expression; synthetic peptide; tissue /cell culture; transfection; virus receptors

DESCRIPTION: This project focuses on a G protein-coupled receptor of HHV-8 which was found to be associated with Kaposi's sarcoma. It has recently been shown that this receptor functions via the phosphoinositide-inositol 1,4,5-triphosphate-calcium-protein kinase C signal transduction pathway, to induce cell proliferation and transformation; suggesting that HHV-8/GPCR may play a necessary, if not sufficient role in the abnormal proliferation exhibited by KS cells or lymphomatous B cells. The objectives of this research are to understand the biology of HHV-8 GPCR, and its role in the pathogenesis of KS. Three specific aims are proposed. The first is to delineate the signaling mechanisms and modes of potential regulation of HHV-8 GPCR, and the biological consequences of HHV-8 GPCR expression in various cell types, such as cos, 293T, NIH 3T3, epithelial and B cells. The second is to discover high affinity, specific ligands for HHV-8 GPCR based on analogy to known chemokines. The third is to determine the roles of HHV-8 GPCR in disease pathogenesis.

描述：本项目主要研究HHV-8的G蛋白偶联受体