RNA splicing control of meristem identity, cell division and differentiation in Arabidopsis.

RNA剪接控制拟南芥分生组织特性、细胞分裂和分化。

• 批准号: BB/S000305/1
• 负责人: Keith Lindsey
• 金额: $ 50.54万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS000305%2F1
• 关键词: RNA; splicing; control; meristem; identity

Plants and animals control their development, from single cell to complex multicellular organism, by regulating the activities of thousands of genes. Each gene produces an RNA molecule, and at one time it was thought that one RNA molecule contains the information to produce one protein. It has been discovered in recent years that complex organisms can in fact splice sections of a given RNA molecule, to generate alternative versions of the final, mature RNA, and this in turn means that a greater diversity of proteins can be produced from a single gene. This production of a range of spliced versions of RNAs is called alternative splicing, and contributes to the fine level of control to regulate the activities of cells. The mechanism and role of alternative splicing in specific developmental processes is still, however, at a very early stage. We have identified a plant gene, termed MDF, for which we have new information indicating a novel role in regulating RNA splicing to regulate cell identitity and cell division activity in the growing regions of plants, the apical meristems. The project aims to understand which RNAs are spliced by MDF, and how these target RNAs contribute to the complex network of genes regulating the activity of meristems.

植物和动物通过调节数千个基因的活动来控制它们从单细胞到复杂的多细胞生物体的发育。每个基因产生一个RNA分子，曾经有人认为一个RNA分子包含产生一种蛋白质的信息。近年来人们发现，复杂的生物体实际上可以拼接给定RNA分子的部分，以产生最终成熟RNA的替代版本，这反过来意味着可以从单个基因中产生更大多样性的蛋白质。这种产生一系列剪接形式的RNA被称为选择性剪接，并有助于精细的控制水平，以调节细胞的活动。然而，选择性剪接在特定发育过程中的机制和作用仍处于非常早期的阶段。我们已经确定了一个植物基因，称为p21，我们有新的信息表明，在调节RNA剪接，以调节细胞的身份和细胞分裂活性在植物的生长区域，顶端分生组织的新作用。该项目的目的是了解哪些RNA被剪接，以及这些靶RNA如何有助于调节分生组织活性的复杂基因网络。