PATHOGENESIS OF MESENCHYMAL TUMORS INDUCED BY ASBESTOS

石棉诱发的间质肿瘤的发病机制

• 批准号: 3251300
• 负责人: Agnes B Kane
• 金额: $ 12.43万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-15 至 1988-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3251300
• 关键词: asbestos; chemical related neoplasm /cancer; disease /disorder model; electron microscopy; environment related neoplasm /cancer; fluorescence spectrometry; histochemistry /cytochemistry; immunofluorescence technique; lymph nodes; macrophage; macrophage activating factor; mesothelioma; model design /development; neoplasm /cancer immunology; peritoneum; tissue /cell culture

Mesotheliomas are hypothesized to develop in response to fibers of critical dimensions. A new model for the induction of mesotheliomas in mice has been developed. Discrete peritoneal nodules develop in response to asbestos, but not to toxic silica particles or nontoxic titanium dioxide particles. Under these conditions, peritoneal macrophages are persistently activated. This model will be used to explore the histogenesis of mesotheliomas and investigate the role of activated macrophages in the development of these tumors. Energy-dispersive x-ray analysis and electron microscopy will be used to determine whether the surface or size of asbestos fibers become modified in vitro. Immunocytochemical typing of intermediate filaments and microspectrofluorimetric assay of DNA content will be used to identify possible precursor lesions of mesotheliomas. Finally, the role of activated macrophages in stimulating proliferation of possible precursor populations and mesotheliomas will be evaluated.

间皮瘤被假设为发展中国家的纤维的关键 尺寸. 一种新的小鼠间皮瘤诱导模型， 开发了 离散的腹膜结节的形成是对 石棉，但不是有毒的二氧化硅颗粒或无毒的二氧化钛 粒子 在这些条件下，腹腔巨噬细胞持续 激活 该模型将用于探讨组织发生的 间皮瘤，并研究活化的巨噬细胞在间皮瘤中的作用。 这些肿瘤的发展。 能量色散X射线分析和电子 显微镜将用于确定是否表面或大小的 石棉纤维在体外被改性。 免疫细胞化学分型 中间丝和DNA含量的显微荧光测定 将用于识别间皮瘤的可能的前驱病变。 最后，活化的巨噬细胞在刺激细胞增殖中的作用， 将评估可能的前体群体和间皮瘤。