BIOCHEMISTRY AND PHARMACOLOGY OF THE RETINA

这是一个研究视网膜生物化学和药理学的建议， 特别强调环核苷酸。 总体目标是确定 cAMP在视网膜功能和代谢中的作用。 我们将解决和 探索视网膜含有环AMP和环GMP系统的假设， 每一个都位于不同的亚细胞或细胞位置， 特点和功能。 利用定量组织化学方法， 技术，我们计划识别，定位和表征鸟苷酸环化酶， 腺苷酸环化酶、环核苷酸磷酸二酯酶和环 视网膜中的核苷酸依赖性蛋白激酶。 区域嘌呤核苷酸 还将评价视网膜中的代谢。 此外，我们希望定义 哪些因素（即，光、神经递质、离子、细胞去极化） 调节视网膜中的环核苷酸水平，并解释其机制。 这 一个将视网膜生物化学和形态学联系起来的项目应该会有所帮助。 解释视觉的分子机制

This is a proposal to study biochemistry and pharmacology of the retina with particular emphasis on cyclic nucleotides. The overall aim is to define the role of cyclic AMP in retinal function and metabolism. We will address and explore the hypothesis that retina contains cyclic AMP and cyclic GMP systems, each in different subcellular or cellular locations and each with different characteristics and functions. Utilizing quantitative histochemical methods and techniques we plan to identify, localize and characterize guanylate cyclases, adenylate cyclases, cyclic nucleotic phosphodiesterases and cyclic nucleotide-dependent protein kinases in retina. Regional purine nucleotide metabolism in retina also will be evaluated. In addition, we wish to define what factors (i.e., light, neurotransmitters, ions, cellular depolarization) regulate cyclic nucleotide levels in retina and explain their mechanisms. This project, which correlates retinal biochemistry and morphology, should help explain some molecular mechanisms underlying vision.