SYNAPTIC ORGANIZATION OF THE OPTIC TECTUM

视顶盖的突触组织

• 批准号: 3255679
• 负责人: JOHN A FREEMAN
• 金额: $ 23.66万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-03-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3255679
• 关键词: cell adhesion; cone cell; electron microscopy; electrophysiology; freeze etching; fresh water environment; gel electrophoresis; histochemistry /cytochemistry; nervous system regeneration; neural information processing; neural transmission; neuroanatomy; neurophysiology; retinal ganglion; superior colliculus; synapses

The principal objective of the proposed research is to identify and characterize some critical cellular mechanisms controlling the growth of optic nerve fibers and the formation of retinotectal synapses, using a combination of morphological, physiological, and biochemical techniques. The retinotectal system provides a very favorable model for studying the dynamics of neurite growth and synaptic development at the cellular and molecular levels, and we plan a set of comparative studies in vivo and in vitro in teleosts (goldfish) and mammals (rats). There are three major goals. The first is to investigate the dynamics of neurite growth in vitro. We have recently discovered that retinal neurite growth cones generate steady currents across their tips, and grow in the direction of an applied field. We plan to examine the effects of local electrical events on cell-substrate adhesion, growth, and the movement and assembly of membrane macromolecules, using internal reflectance and video-enhanced microscopy. Electrogenesis of growing neurites will be monitored with conventional microelectrode recording methods and by the use of a voltage-- sensitive dye. The second goal is to study the development of retinotectal synapses using quantitative EM morphometric and three-dimensional reconstruction methods in order to correlate structural changes and changes in the distribution of membrane macromolecules seen using freeze-fracture methods, with the development of synaptic transmission. The third goal is to establish optimal homogenous culture condtions, by isolating different cell types, and by establishing a continuous cell line of retinal ganglion cells. These studies will involve the development of specific antibodies to different cell types, the production of which would be of great use in the future as tools for cell identification and separation, as molecular probes for membrane macromolecules, and in providing an animal model for autoimmune retinopathies. These studies embrace an area of research (retinal regeneration) targeted by a recent report of the National Advisory Eye Council as being of special interest.

拟议研究的主要目标是确定和 描述了一些控制生长的关键细胞机制， 视神经纤维和视网膜顶盖突触的形成，使用 结合形态学、生理学和生物化学技术。 视网膜顶盖系统为研究视网膜的运动提供了一个很好的模型。 神经突生长和突触发育在细胞和 分子水平，我们计划在体内和体内进行一系列比较研究， 在硬骨鱼（金鱼）和哺乳动物（大鼠）的体外。 有三大 目标. 第一个是研究神经突起生长的动力学， 体外 我们最近发现视网膜神经突生长锥 在它们的尖端产生稳定的电流，并朝着一个方向生长。 应用领域 我们计划研究局部电流事件的影响 对细胞-基质粘附、生长以及 膜大分子，使用内反射和视频增强 显微镜 将通过以下方式监测生长中的神经突的电发生 传统的微电极记录方法和通过使用电压-- 感光染料 第二个目标是研究视网膜顶盖的发育 突触使用定量EM形态测量和三维 重建方法，以便将结构变化和 在使用冷冻断裂观察到的膜大分子的分布中， 方法，随着突触传递的发展。 第三个目标是 为了建立最佳的均质培养条件，通过分离不同的 细胞类型，并通过建立视网膜神经节细胞系 细胞 这些研究将涉及开发特异性抗体 不同的细胞类型，其生产将是非常有用的， 未来作为细胞鉴定和分离的工具， 膜大分子的探针，并提供动物模型， 自身免疫性视网膜病 这些研究包含了一个研究领域 （视网膜再生）由国家咨询委员会最近的一份报告针对 眼理事会作为特别感兴趣。