权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

EPIDERMAL CELL PROLIFERATION IN SEGMENT MORPHOGENESIS

节段形态发生中的表皮细胞增殖

基本信息

批准号：
3439561
负责人：
JOHN A FREEMAN
金额：
$ 6.39万
依托单位：
UNIVERSITY OF SOUTH ALABAMA
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-06-01 至 1990-08-31
项目状态：
已结题

项目摘要

Growth of organ primordia during post-gastrula morphogenesis, along with cell shape change and cell differentiation, leads to the form and shape of the developing tissue. Although cell replication occurs throughout the organ forming period in most tissues, its role in generating the force for forming the three dimensional shape of the tissue such as the evagination of a tube from an epithelial sheet, is not well understood. Factors that have hindered comprehension of the potential of cell division for shape generation include the previous inability to experimentally separate mitotic pressure from other shape generating processes and the lack of a system in which the process can be followed in situ. In the proposed study the role of cell replication in generating both the number and pattern of cells in the epithelium of a segment, as a stimulant for cell differentiation of specific cell types within the epithelium, and as a motive force in the shape change of the epithelium during limb bud formation will be investigated using larvae of the brine shrimp, Artemia. The simplicity of the tissue organization of these larvae makes this organism ideally suited for this study. The cells involved in the limb bud and segment forming regions are few in number, clearly defined, not surrounded by other cells, and have well timed growth periods. Analysis of the experiments will be carried out using both video-based image analysis and immunocytochemistry. The following hypotheses will be tested: 1) Cell replication occurs in a spatio-temporally directed manner that results in a set pattern of cells and regions of unequal cell density. 2) Attainment of this pattern leads to the differentiation of arthrodial membrane and tendonal cells in which microtubules are involved in cell shape change. 3) Deformation of the epithelium and evagination of the thoracopod limb bud result form unequal cell density, the presence of differentiated arthrodial membrane cells, and region-specific apolysis. 4) Maintenance of the form and shape of the limb bud involves continued patterned cell replication, new cuticle formation, and tendonal cell differentiation. The role of microtubules and microfilaments in the evagination process will also be determined. Some sections of the study will provide preliminary evidence necessary for future studies on regulation of cell replication during development of segmental structures.

原肠胚形态发生后器官原基的生长，随着细胞形状的改变和细胞的分化，导致发育中组织的形态和形状。尽管细胞复制发生在大多数组织的器官形成期，其在生成形成三维空间的力方面的作用组织的形状，如从外翻的管子上皮片，目前还不是很清楚。具有以下特点的因素阻碍了对细胞分裂形状潜力的理解一代人包括之前无法在实验中将有丝分裂压力与其他形状生成过程分开以及缺乏一个可以遵循这一过程的系统 SITE。在拟议的研究中，细胞复制在在上皮中产生细胞的数量和模式作为特定细胞分化的刺激剂上皮内的细胞类型，以及作为肢芽形成过程中上皮细胞的形状变化利用卤虫幼体进行了调查。这个这些幼虫的组织结构的简单性使得非常适合这项研究的生物体。参与该事件的细胞叶芽和节段形成区数量很少，很明显定义好的，没有被其他单元格包围，并且时机很好生长期。将对实验进行分析使用基于视频的图像分析和免疫细胞化学。将检验以下假设：1)细胞复制以时空定向的方式发生，从而导致一组细胞图案和细胞密度不相等的区域。2) 这一模式的实现导致了关节膜和肌腱细胞内有微管参与细胞形状的改变。3)上皮细胞变形与胸足肢芽外翻的结果不相等细胞密度，存在分化的关节膜细胞，以及特定区域的溶栓。4)表格的维护而肢芽的形状涉及到连续的有图案的细胞复制、新角质层形成和腱细胞差异化。微管和微丝在血管紧张性疾病中的作用外溢过程也将被确定。的某些部分这项研究将为未来提供必要的初步证据胚胎发育过程中细胞复制调控的研究进展节段性结构。