RELATIONSHIP OF MAMMALIAN CONES TO PIGMENT EPIHELIUM

哺乳动物视锥细胞与上皮色素的关系

• 批准号: 3256485
• 负责人: DON H ANDERSON
• 金额: $ 16.23万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-09-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3256485
• 关键词: autoradiography; circadian rhythms; cone cell; electron microscopy; eye regeneration; eye surgery; phagocytosis; radiotracer; retina detachment; retina disorder; retinal pigment epithelium; rod cell; visual photoreceptor; visual threshold

Despite their functional and structural similarity, rods and cones also exhibit significant morphological and biochemical differences. These differences almost certainly reflect the cells' specialized functions, but they remain poorly understood. In this project, the cell biology of cone photoreceptors, and their relationship to the retinal pigment epithelium (RPE), is emphasized. Similarities and differences between the two photoreceptor classes will be examined in developing, mature, degenerating, and regenerating photoreceptors using immunocytochemical, autoradiographic, and lectin cytochemical techniques. In addition, changes in the localization and biosynthesis of photoreceptor proteins will be analyzed when the retina is experimentally detached from the RPE. Comparisons of regenerative capacity in primate rod and cone outer segments will be made. Opsin will be localized by immunoelectron microscopy in detached and reattached retinas. Interphotoreceptor retinol binding protein (IRBP) will be localized, and its biosynthesis will be compared in normal and detached retinas. Cellular retinaldehyde binding protein (CRALBP) will be tested for use as a specific cellular marker for migrating and/or proliferating RPE and Muller cells. The biosynthetic pathway for the transport, insertion, and diffusion of cone outer segment membrane proteins will be analyzed using electron microscope autoradiography. Lectin-gold conjugates will be used as ultrastructural probes to identify and quantify binding sites at the photoreceptor-RPE interface. Finally, the distribution of glycoconjugates at the photoreceptor-RPE interface, and their potential role in retinal adhesion, will be examined in retinas treated with a selective inhibitor of glycosylation (tunicamycin-B2).

尽管杆状细胞和锥状细胞的功能和结构相似，但它们也 表现出显着的形态和生化差异。 这些 差异几乎肯定反映了细胞的特殊功能，但是 他们仍然知之甚少。 在这个项目中，锥体的细胞生物学 光感受器及其与视网膜色素上皮的关系 （RPE），被强调。 两者的异同 光感受器类别将在发育中、成熟、退化、 并使用免疫细胞化学、放射自显影技术再生光感受器， 和凝集素细胞化学技术。 此外，变化 将分析光感受器蛋白的定位和生物合成 当视网膜通过实验与 RPE 分离时。 比较 灵长类视杆和视锥外节将具有再生能力。 视蛋白将通过免疫电子显微镜定位在分离和 重新连接视网膜。 光感受器间视黄醇结合蛋白 (IRBP) 是局部化的，其生物合成将在正常和分离状态下进行比较 视网膜。 将测试细胞视黄醛结合蛋白（CRALBP） 用作迁移和/或增殖的特定细胞标记 RPE 和 Muller 细胞。 运输的生物合成途径， 锥体外节膜蛋白的插入和扩散将 使用电子显微镜放射自显影进行分析。 凝集素-金缀合物 将用作超微结构探针来识别和量化结合 位于光感受器-RPE 界面的位点。 最后，分布 光感受器-RPE界面上的糖缀合物及其潜力 在视网膜粘附中的作用，将在经过处理的视网膜中进行检查 选择性糖基化抑制剂（衣霉素-B2）。