OCULAR MECHANISMS OF INFLAMMATORY DISEASES OF THE EYE

不同免疫病理机制在眼部疾病中的作用 是不完全理解的。不同的局部和/或系统细胞和 体液机制可能具有显著不同的作用，无论是单独的还是 与其他机制协同作用，引起眼部炎症和损害 对重要的眼部结构和控制感染性病原体的影响 眼睛。对独立和集体角色的理解 眼部炎症性疾病不同的免疫病理机制 对于将保护与保护分离的最终目标具有重要价值 眼睛免疫反应的破坏性影响。这样的回应是 导致全世界相当大比例失明的原因。现在 研究将集中在免疫病理机制的相互作用上。 由嗜同型细胞(如IgE)抗体、嗜酸性粒细胞和肥大细胞介导 四种眼过敏模型中的细胞：1)实验性春季 外用黄连素致豚鼠结膜炎 荧光素蛋白或蛔虫抗原；2)实验眼 注射钉螺对豚鼠造成的盘尾丝虫病 3)人群对环境抗原的自发超敏反应 特应性狗；4)视网膜色素上皮病变和视网膜变性 与蛔虫感染眼的嗜酸性脉络膜炎有关。这个 免疫操作的影响(例如，路线、时间的变化 以及抗原的剂量，与其他抗原的相伴致敏， 选择性抗炎药物)对皮损发展的影响 将详细定义春季结膜炎模型。局部抗体 生产将在结膜淋巴样细胞的培养中进行研究。这个 特定介质的作用(例如嗜酸性粒细胞趋化因子， 嗜酸性粒细胞和肥大细胞成分)。 蛔虫感染眼的视网膜色素上皮病将在 通过将介体注射到眼内或眼内，以及在 结膜、角膜和视网膜色素培养的体外实验 上皮细胞。抗炎药物和免疫学的能力 影响急性角膜炎症的手法治疗 柠檬酸二乙卡巴拉明治疗实验性眼部盘尾丝虫病 在豚鼠身上会有详细的定义。

The roles of different immunopathologic mechanisms in diseases of the eye are incompletely understood. Distinct local and/or systemic cellular and humoral mechanisms may have significantly different roles, either alone or in concert with other mechanisms, in causing ocular inflammation and damage to vital ocular structures and in controlling infectious agents in the eye. An understanding of the separate and collective roles of the different immunopathologic mechanisms in ocular inflammatory disease would be of great value towards the ultimate goal of separating protective from damaging effects of immune responses in the eye. Such responses are the cause of a substantial proportion of blindness world-wide. The present studies will concentrate on the interactions of immunopathologic mechanisms mediated by homocytotropic (e.g., IgE) antibodies, eosinophils and mast cells in four models of ocular allergy: 1) Experimental vernal conjunctivitis produced in guinea pigs by topical application of fluoresceinylovalbumin or ascarid antigens; 2) Experimental ocular onchocerciasis produced in guinea pigs by the injection of Onchocerca microfilariae; 3) Spontaneous hypersensitivity to environmental antigens in the atopic dog; 4) Retinal pigment epitheliopathy and retinal degeneration associated with an eosinophil choroiditis in ascarid-infected eyes. The effects of immunological manipulations (e.g. variations in route, timing and dosage of antigens, concomitant sensitization with other antigens, selective anti-inflammatory drugs) on the development of lesions in the vernal conjunctivitis model will be defined in detail. Local antibody production will be studied in cultures of conjunctival lymphoid cells. The roles of specific mediators (e.g. eosinophil chemotactic factors, eosinophil and mast cell constituents) in the vernal model, and in the retinal pigment epitheliopathy of ascarid-infected eyes, will be studied in vivo by application of injection of mediators to or into the eye, and in vitro by employing cultures of conjunctival, corneal, and retinal pigmented epithelial cells. The ability of anti-inflammatory drugs and immunological manipulations to affect the acute corneal inflammation caused by diethylcarbazamine citrate treatment of experimental ocular onchocerciasis in guinea pigs will be defined in detail.