OCULAR MECHANISMS OF INFLAMMATORY DISEASES OF THE EYE

眼部炎症性疾病的眼部机制

• 批准号: 3258461
• 负责人: JOHN ROCKEY
• 金额: $ 21.34万
• 依托单位: PRESBYTERIAN MEDICAL CENTER OF PHILA
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-03-01 至 1990-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3258461
• 关键词: active immunization; antiinflammatory agents; cellular immunity; chemoattractants; conjunctivitis; disease /disorder model; electron microscopy; eosinophil; helminthic antigen; histopathology; humoral immunity; immunoglobulin E; immunoglobulin G; immunologic techniques; immunopathology; inflammation; keratitis; mast cell; microscopy; monoclonal antibody; parasitic eye infections; passive immunization; retina degeneration; retinal pigment epithelium; tissue /cell culture

The roles of different immunopathologic mechanisms in diseases of the eye are incompletely understood. Distinct local and/or systemic cellular and humoral mechanisms may have significantly different roles, either alone or in concert with other mechanisms, in causing ocular inflammation and damage to vital ocular structures and in controlling infectious agents in the eye. An understanding of the separate and collective roles of the different immunopathologic mechanisms in ocular inflammatory disease would be of great value towards the ultimate goal of separating protective from damaging effects of immune responses in the eye. Such responses are the cause of a substantial proportion of blindness world-wide. The present studies will concentrate on the interactions of immunopathologic mechanisms mediated by homocytotropic (e.g., IgE) antibodies, eosinophils and mast cells in four models of ocular allergy: 1) Experimental vernal conjunctivitis produced in guinea pigs by topical application of fluoresceinylovalbumin or ascarid antigens; 2) Experimental ocular onchocerciasis produced in guinea pigs by the injection of Onchocerca microfilariae; 3) Spontaneous hypersensitivity to environmental antigens in the atopic dog; 4) Retinal pigment epitheliopathy and retinal degeneration associated with an eosinophil choroiditis in ascarid-infected eyes. The effects of immunological manipulations (e.g. variations in route, timing and dosage of antigens, concomitant sensitization with other antigens, selective anti-inflammatory drugs) on the development of lesions in the vernal conjunctivitis model will be defined in detail. Local antibody production will be studied in cultures of conjunctival lymphoid cells. The roles of specific mediators (e.g. eosinophil chemotactic factors, eosinophil and mast cell constituents) in the vernal model, and in the retinal pigment epitheliopathy of ascarid-infected eyes, will be studied in vivo by application of injection of mediators to or into the eye, and in vitro by employing cultures of conjunctival, corneal, and retinal pigmented epithelial cells. The ability of anti-inflammatory drugs and immunological manipulations to affect the acute corneal inflammation caused by diethylcarbazamine citrate treatment of experimental ocular onchocerciasis in guinea pigs will be defined in detail.

不同免疫病理机制在眼部疾病中的作用 并不完全理解。 不同的局部和/或全身细胞和 体液机制可能具有显著不同的作用，单独或 与其他机制一起引起眼部炎症和损伤 重要的眼部结构，并在控制传染性病原体在 眼睛 对联合国各机构的单独和集体作用的理解 眼部炎症性疾病的不同免疫病理机制将 对实现保护性与非保护性分离的最终目标具有重要价值。 免疫反应对眼睛的破坏作用。 这些反应是 全世界相当大一部分失明的原因。 本 研究将集中在免疫病理机制的相互作用 由亲同型细胞性（例如，IgE）抗体、嗜酸性粒细胞和肥大细胞 细胞在四种眼变态反应模型中：1）实验性春季 豚鼠局部应用 荧光素清蛋白或蛔虫抗原; 2）实验性眼 通过注射盘尾丝虫在豚鼠中产生的盘尾丝虫病 微丝蚴; 3）对环境抗原的自发超敏反应， 4）视网膜色素上皮病变和视网膜变性 与蛔虫感染眼的嗜酸性脉络膜炎相关。 的 免疫操作的影响（例如，途径、时机的变化 和抗原的剂量，与其它抗原的伴随致敏， 选择性抗炎药）对病变发展的影响 将详细定义春季结膜炎模型。 局部抗体 将在结膜淋巴样细胞的培养物中研究产生。 的 特异性介质（例如嗜酸性粒细胞趋化因子， 嗜酸性粒细胞和肥大细胞成分）在春季模型，并在 视网膜色素上皮病变的蛔虫感染的眼睛，将研究 通过将介质注射到眼或眼内， 通过使用结膜、角膜和视网膜色素沉着的培养物进行体外培养， 上皮细胞 抗炎药和免疫抑制剂的作用 手法影响急性角膜炎症引起的 枸橼酸二乙卡巴肼治疗实验性眼盘尾丝虫病 将详细定义豚鼠。