Cytek Aurora Spectral Analyser: essential investment to expand the Babraham Institute Flow Cytometry capabilities, enabling pioneering bioscience

Cytek Aurora 光谱分析仪：扩展 Babraham 研究所流式细胞术能力的必要投资，实现开创性的生物科学

• 批准号: BB/S019189/1
• 负责人: Adrian Liston
• 金额: $ 41.97万
• 依托单位: Babraham Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS019189%2F1
• 关键词: Cytek; Aurora; Spectral; Analyser; essential

The field of flow cytometry is essential in many areas of life science research, such as immunology, haematology, cell biology and cancer studies. At BI, we study healthy ageing and Flow Cytometry is an essential tool to do this. The Flow Cytometry Core Facility is a busy, successful facility housing state of the art cytometers that are supported by dedicated and experienced staff. The Flow Cytometry Core prides itself on providing excellent service to BI scientists (over 100 use the facility) and 17 bio-tech companies who are based at Babraham Research Campus. It is important that the equipment within the Flow Cytometry Facility is state-of-the-art to facilitate the pioneering science carried out by BI Scientists. There have been huge advances in the technology behind the flow cytometry instruments and the fluorochromes that are measured on the instruments and therefore it is important that BI Flow Cytometry Facility keeps up to date with these technology changes. One of the biggest recent changes in technology has been the introduction of spectral analysers. This equipment grant is for a Cytek Bioscience 5-laser Aurora Spectral Analyser to be housed within the BI Flow Cytometry Facility. The Cytek Bioscience Aurora Spectral Analyser (hereafter 'Aurora'), a new spectral analyser which looks at the spectra of fluorochromes across each of its 5 lasers. This technology has been used in the field of microscopy for years but is new to flow cytometry. The analysers currently within the Core can theoretically measure 27 fluorescent markers, however it is difficult to combine more than 20 markers together due to the lack of unique commercially available dyes and the challenges of using a number of markers in combination. The Aurora allows up to 50 fluorescent parameters to be used including combinations of dyes that are impossible in conventional flow cytometry. This means that fluorochromes with similar excitation and emission maximums can be used together in experiments. This ultimately enables scientists to look at more markers from a sample, allowing them to study individual cells in more detail, reducing the size of samples needed for analysis. Another challenge when studying cells using flow cytometry can be strong cellular autofluorescence which means it can be difficult to measure and quantify dim fluorescent markers. The Aurora measures autofluorescence as a separate parameter. Autofluorescence can then be subtracted from the fluorescence allowing scientists to detect and quantify dim fluorescent signals. Increasing the multicolour capabilities of the BI Flow Cytometry Core Facility is essential to continue the BI world-class science. The addition of an Aurora will strengthen the capabilities of the Flow Cytometry Core Facility, which will in turn help BI scientists with their bioscience research into lifelong health and wellbeing, particularly in the areas of ageing cell immunology and regenerative medicine. Cutting-edge flow cytometry capabilities also boost our connections with Babraham Research Campus and other scientists and maximises the value of the invested equipment funding.

流式细胞术在生命科学研究的许多领域都是必不可少的，如免疫学、血液学、细胞生物学和癌症研究。在BI，我们研究健康老龄化，流式细胞术是实现这一目标的重要工具。流式细胞仪核心设施是一个忙碌、成功的设施，拥有最先进的细胞仪，并由专业且经验丰富的工作人员提供支持。流式细胞仪核心为BI科学家（超过100人使用该设施）和位于Babraham研究园区的17家生物技术公司提供优质服务而自豪。重要的是，流式细胞仪设施内的设备是最先进的，以促进BI科学家开展的开创性科学。流式细胞术仪器背后的技术和仪器上测量的荧光染料已经取得了巨大的进步，因此BI流式细胞术机构必须跟上这些技术变化。最近最大的技术变化之一是光谱分析仪的引入。该设备授权用于将Cytek Bioscience 5激光Aurora光谱分析仪安装在BI流式细胞仪设施内。 Cytek Bioscience Aurora光谱分析仪（以下简称“Aurora”）是一种新的光谱分析仪，可以通过其5个激光器中的每一个来观察荧光染料的光谱。这项技术已在显微镜领域使用多年，但对流式细胞术来说是新的。目前在核心内的分析仪理论上可以测量27种荧光标记物，但是由于缺乏独特的市售染料和组合使用许多标记物的挑战，很难将20多种标记物联合收割机组合在一起。Aurora允许使用多达50种荧光参数，包括常规流式细胞术中不可能使用的染料组合。这意味着具有相似激发和发射最大值的荧光染料可以在实验中一起使用。这最终使科学家能够从一个样本中观察更多的标记物，使他们能够更详细地研究单个细胞，减少分析所需的样本大小。 使用流式细胞术研究细胞时的另一个挑战可能是强烈的细胞自发荧光，这意味着难以测量和量化暗淡的荧光标记物。Aurora测量自发荧光作为单独的参数。然后可以从荧光中减去自体荧光，使科学家能够检测和量化暗淡的荧光信号。 提高BI流式细胞仪核心设施的多色能力对于继续BI世界级科学至关重要。Aurora的加入将加强流式细胞仪核心设施的能力，这将反过来帮助BI科学家进行终身健康和福祉的生物科学研究，特别是在衰老细胞免疫学和再生医学领域。尖端的流式细胞仪功能还促进了我们与Babraham研究园区和其他科学家的联系，并最大限度地提高了投资设备资金的价值。

项目成果

Atypical B cells and impaired SARS-CoV-2 neutralization following heterologous vaccination in the elderly.

• DOI: 10.1016/j.celrep.2023.112991
• 发表时间: 2023-08
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: I. Ferreira;C. Q. Lee;W. Foster;A. Abdullahi;L. Dratva;Z. Tuong;B. Stewart;J. Ferdinand;Stephane M. Guillaume;Martin Potts;Marianne R Perera;Ben Krishna;Ana Ivis Peñalver;Mia Cabantous;S. Kemp;L. Ceron-Gutierrez;S. Ebrahimi;P. Lyons;Ken G. C. Smith;J. Bradley;D. Collier;L. McCoy;A. A. van der Klaauw-A.;James E. D. Thaventhiran;I. Farooqi;Sarah A. Teichmann;P. MacAry;R. Doffinger;M. Wills;M. Linterman;M. Clatworthy;Ravindra K. Gupta

AutoSpill is a principled framework that simplifies the analysis of multichromatic flow cytometry data.

Autospill是一个原则性的框架，可简化多种流式细胞仪数据的分析。

• DOI: 10.1038/s41467-021-23126-8
• 发表时间: 2021-05-17
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Roca CP;Burton OT;Gergelits V;Prezzemolo T;Whyte CE;Halpert R;Kreft Ł;Collier J;Botzki A;Spidlen J;Humblet-Baron S;Liston A
• 通讯作者: Liston A

Tfh cells and the germinal center are required for memory B cell formation & humoral immunity after ChAdOx1 nCoV-19 vaccination.

• DOI: 10.1016/j.xcrm.2022.100845
• 发表时间: 2022-12-20
• 期刊: CELL REPORTS MEDICINE
• 影响因子: 14.3
• 作者: Foster, William S.;Lee, Jia Le;Thakur, Nazia;Newman, Joseph;Spencer, Alexandra J.;Davies, Sophie;Woods, Danielle;Godfrey, Leila;Hay, Iain M.;Innocentin, Silvia;Yam-Puc, Juan Carlos;Horner, Emily C.;Sharpe, Hayley J.;Thaventhiran, James E.;Bailey, Dalan;Lambe, Teresa;Linterman, Michelle A.
• 通讯作者: Linterman, Michelle A.

Do more with Less: Improving High Parameter Cytometry Through Overnight Staining.

• DOI: 10.1002/cpz1.589
• 发表时间: 2022-11
• 期刊: Current protocols
• 作者: Whyte, Carly E.;Tumes, Damon J.;Liston, Adrian;Burton, Oliver T.
• 通讯作者: Burton, Oliver T.

A Cross Entropy Test Allows Quantitative Statistical Comparison of t-SNE and UMAP Representations

交叉熵测试允许对 t-SNE 和 UMAP 表示进行定量统计比较

• DOI: 10.2139/ssrn.4189024
• 发表时间: 2022
• 期刊: SSRN Electronic Journal
• 作者: Roca C