Cytek Aurora Spectral Analyser: essential investment to expand the Babraham Institute Flow Cytometry capabilities, enabling pioneering bioscience

Cytek Aurora 光谱分析仪：扩展 Babraham 研究所流式细胞术能力的必要投资，实现开创性的生物科学

• 批准号: BB/S019189/1
• 负责人: Adrian Liston
• 金额: $ 41.97万
• 依托单位: Babraham Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS019189%2F1
• 关键词: Cytek; Aurora; Spectral; Analyser; essential

The field of flow cytometry is essential in many areas of life science research, such as immunology, haematology, cell biology and cancer studies. At BI, we study healthy ageing and Flow Cytometry is an essential tool to do this. The Flow Cytometry Core Facility is a busy, successful facility housing state of the art cytometers that are supported by dedicated and experienced staff. The Flow Cytometry Core prides itself on providing excellent service to BI scientists (over 100 use the facility) and 17 bio-tech companies who are based at Babraham Research Campus. It is important that the equipment within the Flow Cytometry Facility is state-of-the-art to facilitate the pioneering science carried out by BI Scientists. There have been huge advances in the technology behind the flow cytometry instruments and the fluorochromes that are measured on the instruments and therefore it is important that BI Flow Cytometry Facility keeps up to date with these technology changes. One of the biggest recent changes in technology has been the introduction of spectral analysers. This equipment grant is for a Cytek Bioscience 5-laser Aurora Spectral Analyser to be housed within the BI Flow Cytometry Facility. The Cytek Bioscience Aurora Spectral Analyser (hereafter 'Aurora'), a new spectral analyser which looks at the spectra of fluorochromes across each of its 5 lasers. This technology has been used in the field of microscopy for years but is new to flow cytometry. The analysers currently within the Core can theoretically measure 27 fluorescent markers, however it is difficult to combine more than 20 markers together due to the lack of unique commercially available dyes and the challenges of using a number of markers in combination. The Aurora allows up to 50 fluorescent parameters to be used including combinations of dyes that are impossible in conventional flow cytometry. This means that fluorochromes with similar excitation and emission maximums can be used together in experiments. This ultimately enables scientists to look at more markers from a sample, allowing them to study individual cells in more detail, reducing the size of samples needed for analysis. Another challenge when studying cells using flow cytometry can be strong cellular autofluorescence which means it can be difficult to measure and quantify dim fluorescent markers. The Aurora measures autofluorescence as a separate parameter. Autofluorescence can then be subtracted from the fluorescence allowing scientists to detect and quantify dim fluorescent signals. Increasing the multicolour capabilities of the BI Flow Cytometry Core Facility is essential to continue the BI world-class science. The addition of an Aurora will strengthen the capabilities of the Flow Cytometry Core Facility, which will in turn help BI scientists with their bioscience research into lifelong health and wellbeing, particularly in the areas of ageing cell immunology and regenerative medicine. Cutting-edge flow cytometry capabilities also boost our connections with Babraham Research Campus and other scientists and maximises the value of the invested equipment funding.

流式细胞仪领域在生命科学研究的许多领域至关重要，例如免疫学，血液学，细胞生物学和癌症研究。在BI，我们研究健康的衰老和流式细胞仪是这样做的必要工具。流式细胞仪核心设施是一个繁忙，成功的设施住房状态，其最先进的细胞仪是由专门和经验丰富的员工支持的。流式细胞仪核心以为BA Babraham Research Campus的BAICOCICONS和17家​​生物技术公司提供出色的服务而自豪。重要的是，流式细胞仪设施中的设备是最先进的，以促进BI科学家进行的开创性科学。流式细胞仪仪器和在该仪器上测量的荧光色素的技术方面取得了巨大进步，因此，重要的是，BI流式细胞仪设施与这些技术变化保持最新状态非常重要。最近，技术最大的变化之一是引入光谱分析仪。该设备赠款是供Cytek Bioscience 5-laser Aurora光谱分析仪安装在BI流式细胞仪设施中的。 Cytek Bioscience Aurora光谱分析仪（以下称“ Aurora”），这是一种新的光谱分析仪，该分析仪着眼于其5个激光器中的每个激光器中的荧光色素光谱。多年来，该技术已在显微镜领域中使用，但对于流式细胞仪而言是新的。当前核心内的分析仪理论上可以测量27个荧光标记，但是由于缺乏独特的市售染料以及组合使用许多标记的挑战，很难将20多个标记组合在一起。 Aurora允许使用多达50个荧光参数，包括在常规流式细胞仪中不可能的染料组合。这意味着在实验中可以一起使用具有相似激发和发射最大值的荧光染料。这最终使科学家能够查看样本中的更多标记，使他们能够更详细地研究单个细胞，从而减少分析所需的样品的大小。 使用流式细胞仪研究细胞时的另一个挑战可能是强细胞自荧光，这意味着很难测量和量化昏暗的荧光标记。 Aurora将自动荧光作为单独参数测量。然后可以从荧光中减去自荧光，从而使科学家检测和量化昏暗的荧光信号。 提高BI流式细胞仪核心设施的多色功能对于继续BI世界一流的科学至关重要。 Aurora的添加将增强流式细胞仪核心设施的能力，这反过来将帮助BI科学家对终身健康和福祉的生物科学研究，尤其是在老化的细胞免疫学和再生医学领域。尖端流式细胞仪的能力还可以提高我们与Babraham研究校园和其他科学家的联系，并最大程度地提高投资设备资金的价值。

项目成果

Atypical B cells and impaired SARS-CoV-2 neutralization following heterologous vaccination in the elderly.

• DOI: 10.1016/j.celrep.2023.112991
• 发表时间: 2023-08
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: I. Ferreira;C. Q. Lee;W. Foster;A. Abdullahi;L. Dratva;Z. Tuong;B. Stewart;J. Ferdinand;Stephane M. Guillaume;Martin Potts;Marianne R Perera;Ben Krishna;Ana Ivis Peñalver;Mia Cabantous;S. Kemp;L. Ceron-Gutierrez;S. Ebrahimi;P. Lyons;Ken G. C. Smith;J. Bradley;D. Collier;L. McCoy;A. A. van der Klaauw-A.;James E. D. Thaventhiran;I. Farooqi;Sarah A. Teichmann;P. MacAry;R. Doffinger;M. Wills;M. Linterman;M. Clatworthy;Ravindra K. Gupta

AutoSpill is a principled framework that simplifies the analysis of multichromatic flow cytometry data.

• DOI: 10.1038/s41467-021-23126-8
• 发表时间: 2021-05-17
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Roca CP;Burton OT;Gergelits V;Prezzemolo T;Whyte CE;Halpert R;Kreft Ł;Collier J;Botzki A;Spidlen J;Humblet-Baron S;Liston A
• 通讯作者: Liston A

Tfh cells and the germinal center are required for memory B cell formation & humoral immunity after ChAdOx1 nCoV-19 vaccination.

• DOI: 10.1016/j.xcrm.2022.100845
• 发表时间: 2022-12-20
• 期刊: CELL REPORTS MEDICINE
• 影响因子: 14.3
• 作者: Foster, William S.;Lee, Jia Le;Thakur, Nazia;Newman, Joseph;Spencer, Alexandra J.;Davies, Sophie;Woods, Danielle;Godfrey, Leila;Hay, Iain M.;Innocentin, Silvia;Yam-Puc, Juan Carlos;Horner, Emily C.;Sharpe, Hayley J.;Thaventhiran, James E.;Bailey, Dalan;Lambe, Teresa;Linterman, Michelle A.
• 通讯作者: Linterman, Michelle A.

Do more with Less: Improving High Parameter Cytometry Through Overnight Staining.

• DOI: 10.1002/cpz1.589
• 发表时间: 2022-11
• 期刊: Current protocols
• 作者: Whyte, Carly E.;Tumes, Damon J.;Liston, Adrian;Burton, Oliver T.
• 通讯作者: Burton, Oliver T.

A Cross Entropy Test Allows Quantitative Statistical Comparison of t-SNE and UMAP Representations

交叉熵测试允许对 t-SNE 和 UMAP 表示进行定量统计比较

• DOI: 10.2139/ssrn.4189024
• 发表时间: 2022
• 期刊: SSRN Electronic Journal
• 作者: Roca C