GRADED HYPOXIA AND PHOTORECEPTOR-RPE INTERACTIONS
分级缺氧和光感受器-RPE 相互作用
基本信息
- 批准号:3261547
- 负责人:
- 金额:$ 4.53万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1985
- 资助国家:美国
- 起止时间:1985-09-30 至 1988-09-29
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The overall objective of this proposal is to learn the mechanisms by which
graded, and in particular, mild hypoxia alters the functional interactions
between the neural retina and the retinal pigment epithelium (RPE). Recent
experiments in the intact cat retina and in my preliminary in vitro results
have demonstrated that quite mild levels of hypoxia affect potassium
homeostasis in the subretinal space and the potentials of the RPE. In
vitro models of the gecko retina will be developed to investigate the
origins and cellular mechanisms of these hypoxic effects.
Specific experiments will determine the relative roles of photoreceptors,
RPE and Muller cells in 1) a hypoxia-induced increase in steady-state
potassium in the dark; 2) hypoxia-induced changes in the amplitude and
time-course of light-evoked potassium responses; and 3) hypoxia-induced
changes in apical and basal membrane potentials and conductances both in
the dark and in response to light.
The strategy will be to study hypoxic responses in the isolated retina and
isolated RPE preparations to distinguish effects on photoreceptor and RPE
cells. Intracellular recordings will be used to record effects of hypoxia
on membrane potential and conductance; potassium-selective microelectrodes
will be used to explore effects of hypoxia on potassium. A new
electrophysiological technique has been developed to study the RPE
sodium-potassium pump.
The gecko retina has been shown to be a good model for normoxic responses
from the intact cat eye. Since the cat is a good model for human, results
form this in vitro hypoxic model should apply to human. Little is known
about the primary effects of graded hypoxia on neurons or epithelia in
general, or specifically on the photoreceptors and RPE, so that the
proposed research will be of significance to both basic and clinical
science.
这项建议的总体目标是了解通过哪些机制
渐进性的,特别是轻度缺氧改变了功能的相互作用
位于神经视网膜和视网膜色素上皮(RPE)之间。近期
在完整的猫视网膜和我的初步体外结果中进行的实验
已经证明,相当轻微的低氧水平会影响钾
视网膜下间隙的动态平衡和RPE的电位。在……里面
将建立壁虎视网膜的体外模型,以研究
这些低氧效应的起源和细胞机制。
具体的实验将确定光感受器的相对作用,
RPE和Muller细胞在1)低氧诱导的稳态增加
黑暗中的钾;2)低氧引起的波幅和
光诱导钾反应的时程;3)低氧诱导
大鼠根尖和基底膜电位及电导的变化
黑暗和对光的反应。
该策略将研究离体视网膜的缺氧反应,并
分离的RPE制剂对光感受器和RPE的影响
细胞。细胞内记录将被用来记录低氧的影响
膜电位和电导的研究;钾选择微电极
将被用来探索低氧对钾的影响。一种新的
电生理技术已被发展用于RPE的研究
钠钾泵。
壁虎视网膜已被证明是常氧性反应的良好模型。
来自完好无损的猫眼。由于猫是人类的好模型,结果是
这种体外低氧模型应适用于人。鲜为人知
关于分级低氧对神经细胞或上皮细胞的初级影响
一般的,或专门针对光感受器和RPE的,所以
拟议的研究将对基础和临床都具有重要意义
科学。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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EDWIN R GRIFF其他文献
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{{ truncateString('EDWIN R GRIFF', 18)}}的其他基金
GRADED HYPOXIA AND PHOTORECEPTOR-RPE INTERACTIONS
分级缺氧和光感受器-RPE 相互作用
- 批准号:
3261548 - 财政年份:1985
- 资助金额:
$ 4.53万 - 项目类别:
GRADED HYPOXIA AND PHOTORECEPTOR-RPE INTERACTIONS
分级缺氧和光感受器-RPE 相互作用
- 批准号:
3261544 - 财政年份:1985
- 资助金额:
$ 4.53万 - 项目类别:
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