PHYSIOLOGY AND PHARMACOLOGY OF RETINAL BIPOLAR CELLS

这项拨款旨在研究生理和药理学 金鱼双极细胞的特性。 单个细胞，从 金鱼视网膜，并保持在组织培养，将研究使用 新开发的膜片钳记录方法。 这些方法使得 对细胞膜中单个通道的研究。 它们还提供了一种 用于记录和电压箝位小单元。 他们可以提供 关于神经膜生理学的信息， 比以前更丰富和详细。 我们要用这些 技术来研究双极细胞，因为这些视网膜神经元是 在视觉信号处理中至关重要，因为很少 了解其功能。 我们实验的目的是 提供有关基本膜性质的基本知识， 双极细胞：它们的膜电阻和电容， 它们的静息电位，以及电压依赖性和其他 双极电池中存在的电导。 我们还将调查 药理学试剂的作用，特别是推定的 神经递质物质在孤立的双极细胞。 这样我们可以 研究这些药物对双极细胞本身的影响， 没有与其他细胞连接所产生的并发症 类型 除了提供有关生理学的基本信息外， 脊椎动物视网膜，我们也希望提供有用的知识， 视网膜细胞的药理学，这可能有助于评估 药物对视力和其他视力指标的影响 功能

This grant proposes to study the physiological and pharmacological properties of goldfish bipolar cells. Single cells, dissociated from goldfish retina and maintained in tissue culture, will be studied using newly-developed patch-clamp recording methods. These methods make possible the study of single channels in cell membranes. They also provide a means for recording from and voltage clamping small cells. They can provide information about the physiology of nerve membranes in much greater abundance and detail than was previously possible. We shall use these techniques to study bipolar cells, since these retinal neurons are of essential importance in the processing of visual signals, and since little is known about their function. The purpose of our experiments is to provide fundamental knowledge about the basic menbrane properties of bipolar cells: their membrane resistance and capacitance, the nature of their resting potentials, and the function of voltage-dependent and other conductances present in the bipolar cells. We shall also investigate the effects of pharmacological agents, and in particular of putative neurotransmitter substances on isolated bipolar cells. In this way we can investigate the effects of these drugs on the bipolar cells themselves, free of the complications produced by their connections to other cell types. In addition to providing basic information about the physiology of vertebrate retina, we hope also to provide useful knowledge about the pharmacology of retinal cells, which may assist in the assessment of the effects of pharmacological agents on acuity and other measures of visual function.