EYE INFECTION OF CYTOMEGALOVIRUS

人类巨细胞病毒(CMV)的许多眼部疾病 眼部机会性感染，主要发生在 免疫抑制的个体。作为免疫抑制的数量 近年来患者的增加主要是由于后天获得性的 免疫缺陷综合征(AIDS)，巨细胞病毒已成为病原体 治疗一种重要的眼疾。然而，巨细胞病毒眼的发病机制 目前对感染的了解很少。鉴于十个严重的 巨细胞病毒眼部感染的性质及其发病率的增加 艾滋病患者眼部感染的关键评估 巨细胞病毒在人眼感染中的作用及其综合研究 迫切需要了解这种疾病的性质。 小鼠巨细胞病毒眼部感染的初步研究 表明与人类一样，完整的宿主细胞免疫是一种 保护小鼠免受感染的重要因素 和MCMV的复发。因此，作为我们最初的努力， 评价宿主细胞免疫在发病机制中的作用 关于CMV眼部感染，以下一系列实验是 建议： 1)小鼠各种细胞免疫成分的作用，即 辅助T细胞(L3T4细胞)、抑制性T细胞(Lyt-2细胞)、 自然杀伤细胞和巨噬细胞在诱导病毒血症中的作用 而MCMV全身感染期间的原发眼部感染将 被研究。 2)眼和巨噬细胞中MCMV潜伏期的建立 将对以下初次感染进行检查，以及 眼睛组织中的病毒基因组将通过 共培养方法和DNA-DNA杂交技术与 MCMV-DNA探针。 3)体内可能起作用的细胞免疫成分 眼睛中潜伏的MCMV的重新激活将通过以下方式确定 评价每种免疫选择性耗竭的效果 关于潜伏病毒重新激活的组件。 拟议的研究将为我们提供迄今无法获得的信息 关于CMV眼部感染的研究将对我们的 了解该病的发病机制，也将有助于 我们正在寻找有效的预防和预防手段 人类巨细胞病毒感染的治疗。

Many ocular diseases of cytomegalovirus (CMV) in humans are opportunistic infections of the eye and occur predominantly in immunosuppressed individuals. As the number of immunosuppressed patients increases in recent years due largely to acquired immunodeficiency syndrome (AIDS), CMV has become the causal agent for an important eye disease. Yet, the pathogenesis of CMV eye infection is poorly understood at present. In view of ten serious nature of CMV eye infection and even increasing incidence of CMV eye infection among AIDS patients, a critical assessment of the role of CMV in human eye infection as well as a comprehensive study of the nature of the disease are urgently needed. Our preliminary studies on murine CMV (MCMV) eye infection clearly showed that as in humans, the intact host cellular immunity is an important factor for the protection of the mouse from the infection and recurrence of MCMV. Therefore, as our initial efforts to evaluate the effect of host cellular immunity on the pathogenesis of CMV eye infection, the following series of experiments is proposed: 1) Roles of various cellular immune components of the mouse, namely helper T cells (L3T4+ cells), suppressor T cells (Lyt-2+ cells), natural killer cells and macrophages in the induction of viremia and primary eye infection during systemic infection of MCMV will be studied. 2) The establishment of latency of MCMV in the eye and macrophages following primary infection will be examined, and the location of the virus genomes in the eye tissues will be determined by means of co-culture method and DNA-DNA hybridization techniques with MCMV-DNA probes. 3) Cellular immune components which may be responsible for in vivo reactivation of latent MCMV in the eye will be identified by evaluating the effect of selective depletion of each immune component on the reactivation of the latent virus. The proposed study will provide us hitherto unavailable information on CMV eye infection which will shed some light on our understanding of pathogenesis of the disease and also will assist us in the search for effective means for the prevention and treatment of CMV infections in humans.