EYE INFECTION OF CYTOMEGALOVIRUS

人类巨细胞病毒（CMV）的许多眼部疾病是 眼睛的机会性感染，主要发生在 免疫抑制个体。 由于免疫抑制的数量 近年来，患者增加，主要是由于获得性 免疫缺陷综合征（艾滋病），巨细胞病毒已成为致病因子 一种重要的眼疾 然而，CMV眼的发病机制 目前对感染了解甚少。 鉴于10个严重 CMV眼部感染的性质，甚至增加CMV的发病率 艾滋病患者眼部感染的关键评估 CMV在人类眼部感染中的作用以及一项全面的研究 这种疾病的性质是迫切需要的。 我们对小鼠巨细胞病毒（MCMV）眼部感染的初步研究清楚地表明， 表明，在人类中，完整的宿主细胞免疫是一种 保护小鼠免受感染的重要因素 和MCMV复发。 因此，作为我们的初步努力， 评价宿主细胞免疫在发病机制中的作用 CMV眼部感染，以下一系列实验是 提议： 1)小鼠的各种细胞免疫成分的作用，即 辅助T细胞（L3 T4+细胞），抑制T细胞（Lyt-2+细胞）， 自然杀伤细胞和巨噬细胞在诱导病毒血症中的作用 MCMV全身感染时的原发性眼部感染 被研究。 2)巨噬细胞巨噬细胞在眼及巨噬细胞内潜伏期的建立 将检查原发感染后， 眼组织中的病毒基因组将通过 共培养法和DNA-DNA杂交技术 MCMV-DNA探针。 3)细胞免疫成分可能负责体内 将通过以下方法确定眼睛中潜伏MCMV的再活化： 评价选择性消除每种免疫的效果 对潜伏病毒的再激活的成分。 拟议的研究将为我们提供迄今为止无法获得的信息 关于CMV眼部感染的研究， 了解疾病的发病机制，也将有助于 我们在寻求有效的预防和 治疗人类CMV感染。

Many ocular diseases of cytomegalovirus (CMV) in humans are opportunistic infections of the eye and occur predominantly in immunosuppressed individuals. As the number of immunosuppressed patients increases in recent years due largely to acquired immunodeficiency syndrome (AIDS), CMV has become the causal agent for an important eye disease. Yet, the pathogenesis of CMV eye infection is poorly understood at present. In view of ten serious nature of CMV eye infection and even increasing incidence of CMV eye infection among AIDS patients, a critical assessment of the role of CMV in human eye infection as well as a comprehensive study of the nature of the disease are urgently needed. Our preliminary studies on murine CMV (MCMV) eye infection clearly showed that as in humans, the intact host cellular immunity is an important factor for the protection of the mouse from the infection and recurrence of MCMV. Therefore, as our initial efforts to evaluate the effect of host cellular immunity on the pathogenesis of CMV eye infection, the following series of experiments is proposed: 1) Roles of various cellular immune components of the mouse, namely helper T cells (L3T4+ cells), suppressor T cells (Lyt-2+ cells), natural killer cells and macrophages in the induction of viremia and primary eye infection during systemic infection of MCMV will be studied. 2) The establishment of latency of MCMV in the eye and macrophages following primary infection will be examined, and the location of the virus genomes in the eye tissues will be determined by means of co-culture method and DNA-DNA hybridization techniques with MCMV-DNA probes. 3) Cellular immune components which may be responsible for in vivo reactivation of latent MCMV in the eye will be identified by evaluating the effect of selective depletion of each immune component on the reactivation of the latent virus. The proposed study will provide us hitherto unavailable information on CMV eye infection which will shed some light on our understanding of pathogenesis of the disease and also will assist us in the search for effective means for the prevention and treatment of CMV infections in humans.