FUNCTION OF THE VITRONECTIN RECEPTOR OF HUMAN RPE

人 RPE 玻连蛋白受体的功能

• 批准号: 3265266
• 负责人: SHINTARO SUZUKI
• 金额: $ 16.11万
• 依托单位: DOHENY EYE INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-05-01 至 1992-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3265266
• 关键词: bioassay; cell adhesion; cell biology; extracellular matrix; gene expression; genetic promoter element; genetic regulation; genome; human tissue; laboratory rabbit; messenger RNA; molecular cloning; nucleic acid sequence; physiology; receptor; retinal pigment epithelium; retinaldehyde; transfection

We propose to study ten vitronectin receptor of human retinal pigment epithelial (RPE) cells. The vitronectin receptor is a cell adhesion receptor occupying a vital position between the intracellular and extracellular milieu. One of ten fundamental features of cells from multicellular organisms is their adhesion to the extracellular matrix. Cell adhesion can modulate a number of cellular functions, such as cell growth, cell shape, phagocytosis and cell movement and seems to be involved in many biological processes, including morphogenesis, blood clotting, wound healing and metastasis of malignant cells. Cell adhesion occurs as a result of the interaction between cell surface molecules, known as "cell adhesion receptors", and he cell recognition sites of extracellular matrix proteins. RPE cells proliferate to from epiretinal membranes in pathologic conditions. We postulate that changes in cell adhesion are basic to proliferation and that our studies of vitronectin receptor function may lead to development of a rational treatment for this problem. The molecular properties of the vitronectin receptor are known in detail. We intend to carry out three principal types of functional experiments: (1) examining the properties of the vitronectin receptor in human RPE cells; (2) expressing and repressing the receptor molecules using cDNA constructs with and without modifications, then characterizing the properties of resultant molecules and transfectants; and (3) investigating the gene regulation of the receptor. The information we expect to gather from these experiments will help us to better understand the functional properties and the primary physiological function of the cell adhesion molecules. Such data will also assist us in comprehending the adhesion properties of RPE cells and their function.

我们拟对人视网膜的10种玻连蛋白受体进行研究， 色素上皮（RPE）细胞。 玻连蛋白受体是一种 粘附受体占据着重要的位置， 细胞内和细胞外环境。 细胞的十个基本特征之一， 生物体的主要作用是它们对细胞外基质的粘附。 细胞 粘附可以调节许多细胞功能，例如细胞粘附。 生长、细胞形状、吞噬作用和细胞运动， 参与许多生物过程，包括形态发生， 血液凝固、伤口愈合和恶性细胞转移。 细胞粘附的发生是由于细胞间的相互作用， 表面分子，称为“细胞粘附受体”，而细胞 细胞外基质蛋白的识别位点。 视网膜色素上皮细胞增生形成病理性视网膜前膜 条件 我们假设细胞粘附的变化是基本的 我们对玻连蛋白受体的研究 功能可能会导致合理治疗的发展， 问题. 玻连蛋白受体的分子特性在本发明中是已知的。 详细 我们打算进行三个主要类型的功能 实验：（1）玻连蛋白的性质检测 人RPE细胞中的受体;（2）表达和抑制 受体分子，使用具有和不具有 改性，然后表征所得的性质 分子和转染子;和（3）研究基因 受体的调节。 我们期望从这些实验中收集到的信息将 帮助我们更好地理解功能特性和 细胞粘附分子的主要生理功能。 这些数据也将帮助我们理解粘附性 RPE细胞的特性及其功能。