FUNCTION OF THE VITRONECTIN RECEPTOR OF HUMAN RPE

我们建议研究人视网膜的十种玻璃体连接素受体 色素上皮(RPE)细胞。Vitronectin受体是一种细胞 黏附受体在两个细胞之间占据至关重要的位置 胞内和胞外环境。 来自多细胞的细胞的十个基本特征之一 生物体与细胞外基质的粘附性。细胞 黏附可以调节许多细胞功能，如细胞 生长，细胞形状，吞噬和细胞运动，似乎 参与了许多生物过程，包括形态发生， 血液凝结、伤口愈合和恶性细胞转移。 细胞黏附是细胞间相互作用的结果 被称为“细胞黏附受体”的表面分子和He细胞 细胞外基质蛋白的识别位点。 视网膜色素上皮细胞增殖形成视网膜前膜的病理研究 条件。我们假设细胞黏附的变化是基本的。 对细胞增殖的影响以及我们对Vitronectin受体的研究 功能可能导致开发一种合理的治疗方法 有问题。 玻璃连结蛋白受体的分子特性已知于 细节。我们打算执行三种主要类型的功能 实验：(1)检测玻璃凝集素的性质 人RPE细胞中的受体；(2)表达和抑制 使用带有和不带有cdna结构的受体分子 修饰，然后表征结果的属性 分子和转染体；(3)基因研究 受体的调节。 我们希望从这些实验中收集的信息将 帮助我们更好地理解函数属性和 细胞黏附分子的主要生理功能。 这些数据也将有助于我们理解粘附性。 RPE细胞的特性及其功能。

We propose to study ten vitronectin receptor of human retinal pigment epithelial (RPE) cells. The vitronectin receptor is a cell adhesion receptor occupying a vital position between the intracellular and extracellular milieu. One of ten fundamental features of cells from multicellular organisms is their adhesion to the extracellular matrix. Cell adhesion can modulate a number of cellular functions, such as cell growth, cell shape, phagocytosis and cell movement and seems to be involved in many biological processes, including morphogenesis, blood clotting, wound healing and metastasis of malignant cells. Cell adhesion occurs as a result of the interaction between cell surface molecules, known as "cell adhesion receptors", and he cell recognition sites of extracellular matrix proteins. RPE cells proliferate to from epiretinal membranes in pathologic conditions. We postulate that changes in cell adhesion are basic to proliferation and that our studies of vitronectin receptor function may lead to development of a rational treatment for this problem. The molecular properties of the vitronectin receptor are known in detail. We intend to carry out three principal types of functional experiments: (1) examining the properties of the vitronectin receptor in human RPE cells; (2) expressing and repressing the receptor molecules using cDNA constructs with and without modifications, then characterizing the properties of resultant molecules and transfectants; and (3) investigating the gene regulation of the receptor. The information we expect to gather from these experiments will help us to better understand the functional properties and the primary physiological function of the cell adhesion molecules. Such data will also assist us in comprehending the adhesion properties of RPE cells and their function.