THE TEAR FILM AND THE OCULAR SURFACE: VITAMIN A IN TEAR

拟议工作的总体目标是研究营养 泪液与眼表细胞的关系。 它有 最近有报道说，泪液中含有视黄醇。 由于维生素A 可能影响并参与调节和成熟的细胞在 角膜和结膜，研究将首先集中在交付 维生素A的眼睛表面的眼泪的基础上的假设， 泪液患者的眼表疾病，特别是角化 胶片异常可能部分是由于维生素A输送不足 通过泪液到达眼表上皮。 该项目的具体目标是： 1)高压液相色谱法测定泪液中视黄醇的来源 产生泪液的分泌物和提取物的高效液相色谱（HPLC） 泪腺放射自显影术 用放射性标记的类维生素A治疗动物。 2)研究泪液中维生素A与眼部健康的关系 通过比较角膜的标记， 正常动物和泪液受损动物中放射性标记的类维生素A 功能，通过尝试逆转动物角膜的角化 通过局部应用类维生素A， 用高效液相色谱法测定人和动物泪液中的视黄醇浓度 眼表疾病 3)研究维生素A在角膜中的作用机制，通过研究 类维生素A对培养和体内角膜细胞生长的影响， 并通过HPLC分析角膜中的类维生素A代谢， 局部用类维生素A对内皮创伤愈合的影响。

The overall objective of the proposed work is to study the nutritive relationship of the tears to the cells of the ocular surface. It has recently been reported that retinol is present in tears. Since vitamin A may affect and be involved in the regulation and maturation of cells in the cornea and conjunctiva, the studies will initially focus on delivery of vitamin A to the ocular surface by the tears based on the hypothesis that ocular surface disease, especially keratinization, in patients with tear film abnormalities may in part be due to inadequate delivery of vitamin A to the ocular surface epithelium by the tears. The specific aims of the project are to: 1) determine the source of retinol in the tears by high pressure liquid chromatography (HPLC) of secretions and extracts of the tear-producing glands and by radioautography of tear-producing glands after system treatment of animals with radiolabeled retinoids. 2) study the relationship of vitamin A in tears to the health of the ocular surface by comparing labeling of the cornea by systematically administered radiolabeled retinoids in normal animals and animals with impaired tear function, by attempting to reverse keratinization of corneas in animals with impaired tear function by topical application of retinoids, and by measuring retinol concentration by HPLC in tears of humans and animals with ocular surface disease. 3) study the mechanisms of action of vitamin A in the cornea, by studies of the effect of retinoids on growth of corneal cells in culture and in vivo, and by HPLC analysis of retinoid metabolism in the cornea by studies of the effect of topical retinoids on endothelial wound healing.