OCULAR SURFACE EPITHELIAL CELL CULTURE AND REPLACEMENT

眼表面上皮细胞培养和替换

• 批准号: 3262246
• 负责人: RICHARD A THOFT
• 金额: $ 7.16万
• 依托单位: EYE AND EAR HOSPITAL OF PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 1985-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3262246
• 关键词: cell adhesion; cell motility; conjunctiva; corneal epithelium; corneal stroma; electron microscopy; histochemistry /cytochemistry; histology; human tissue; keratoplasty; membrane activity; microscopy; tissue /cell culture

The aims of this proposal are to establish short-term ocular surface epithelial cell cultures from rabbit and human tissue. The tissues to be studied are corneal epithelium from explants of rabbit and human cornea, and conjunctival epithelium which has resurfaced denuded corneal stromas. Once these cell cultures have been established, they will be characterized in terms of their viability, appearance, both light and electron microscopically and biochemically. Using cells from such cultures, epithelial cell-corneal stroma combinations will be created by incubating corneal stromas in suspensions of these cells. These epithelial cell-stromal cultures will also be characterized, with particular emphasis on cell: substrate interactions, such as spreading, adhesion, and viability. Manipulation of a number of factors, including stromal cell viability, growth factors, serum supplementation and surface active agents (e.g. fibronectins and mucins) will be undertaken to learn more about the nature of cell: substrate interdependencies. Finally, epithelial cell-stromal combination will be used to replace ocular surface components in rabbits, to determine the potential usefulness of this approach in the therapy of ocular surface disease and injury in humans.

本建议的目的是建立短期眼表 来自兔和人组织的上皮细胞培养物。 待处理的组织 研究的是来自兔和人角膜外植体的角膜上皮， 和结膜上皮，其具有重新露出的裸露的角膜基质。 一旦这些细胞培养已经建立，它们将被表征 就它们的活力、外观、光和电子而言， 显微镜下和生物化学上。 使用来自这种培养物的细胞， 将通过在这些悬浮液中孵育角膜基质来产生 细胞 这些上皮细胞-基质培养物也将被表征， 特别强调细胞：基质相互作用，如 铺展、粘附和活性。 一些因素的操纵， 包括基质细胞活力、生长因子、血清补充和 表面活性剂（例如纤连蛋白和粘蛋白）将被用来 了解更多关于细胞的本质：底物相互依赖性。 最后，上皮细胞-基质组合将用于替代眼 表面成分，以确定潜在的有用性， 该方法用于治疗人的眼表面疾病和损伤。