Design and engineering of proteins for biotechnology and biomedicine

用于生物技术和生物医学的蛋白质设计和工程

• 批准号: DP150101371
• 负责人: A/Prof Ashley Buckle
• 金额: $ 24.5万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-01-01 至 2018-12-24
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP150101371
• 关键词: Design; engineering; proteins; biotechnology; biomedicine

The primary aim of this application is to enhance the thermodynamic and folding properties of proteins by redesign and engineering. The structures and folding behaviour of the redesigned proteins will be characterised using X-ray crystallography and biophysical techniques. The expected outcomes of this project are: engineering of new proteins that can be used in biotechnology, medical and pharmaceutical applications, or basic research; fundamental insights into protein design and engineering; and a wealth of knowledge on the factors that dictate protein stability and folding.

该应用程序的主要目的是通过重新设计和工程来增强蛋白质的热力学和折叠特性。重新设计的蛋白质的结构和折叠行为将使用x射线晶体学和生物物理技术进行表征。该项目的预期成果是：可用于生物技术、医疗和制药应用或基础研究的新蛋白质工程；对蛋白质设计和工程的基本见解；以及关于决定蛋白质稳定性和折叠的因素的丰富知识。