SPECTROSCOPIC INVESTIGATIONS OF BIOLOGICAL SYSTEMS

从最广泛的意义上讲，我们的研究目标是了解生物学 物理机制方面的过程和现象，并探讨 使物理现象和技术适应生物学中的问题。 我们面对的更具体的目标和问题 这一建议如下：1.能源转换和能源转换的机制 细菌溶解的蛋白质-叶绿素复合体中的电子转移 膜和蛋白质复合体的表征。不同的物理环境 技术(例如，EPR、光学技术、电子显微镜、穆斯堡尔 光谱学等)和生化方法(例如，免疫学、氨基酸 成分和序列测定，凝胶过滤，凝胶电泳， 等)都被利用了。2.波动技术的应用 从光谱到生物学。在这种技术中，波动(噪声)被用来 获取关于系统的动力学参数(即，速率)的信息 常数)或从大分子的数量波动、分子量 (例如，DNA)可以被确定。3.使用相干光散射来 研究大分子的聚集过程，特别强调 蛋白质结晶机理的研究。

In the broadest sense, our research objectives are to understand biological processes and phenomena in terms of physical mechanisms and to explore the adaptation of physical phenomena and techniques to problems in biology. The more specific goals and problems to which we are addressing ourselves in this proposal are the following: 1. The mechanisms of energy conversion and electron transfer in a protein-chlorophyll complex solubilized from a bacterial membrane and the characterization of the protein complex. Different physical techniques (e.g., EPR, optical techniques, electron microscopy, Mossbauer spectroscopy, etc.) and biochemical methods (e.g., immunological, amino acid composition and sequence determination, gel filtration, gel electrophoresis, etc.) are being used. 2. The application of the technique of fluctuation spectroscopy to biology. In this technique the fluctuations (noise) are used to obtain information about the kinetic parameters of a system (i.e., rate constants) or from the number fluctuations, molecular weights of macromolecules (e.g., DNA) can be determined. 3. The use of coherent light scattering to study aggregation processes of macromolecules with special emphasis on the investigation of crystallization mechanisms of proteins.