SPECTROSCOPIC INVESTIGATIONS OF BIOLOGICAL SYSTEMS

点击翻译按钮获取中文摘要

In the broadest sense, our research objectives are to understand biological processes and phenomena in terms of physical mechanisms and to explore the adaptation of physical phenomena and techniques to problems in biology. The more specific goals and problems to which we are addressing ourselves in this proposal are the following: 1. The mechanisms of energy conversion and electron transfer in a protein-chlorophyll complex solubilized from a bacterial membrane and the characterization of the protein complex. Different physical techniques (e.g., EPR, optical techniques, electron microscopy, Mossbauer spectroscopy, etc.) and biochemical methods (e.g., immunological, amino acid composition and sequence determination, gel filtration, gel electrophoresis, etc.) are being used. 2. The application of the technique of fluctuation spectroscopy to biology. In this technique the fluctuations (noise) are used to obtain information about the kinetic parameters of a system (i.e., rate constants) or from the number fluctuations, molecular weights of macromolecules (e.g., DNA) can be determined. 3. The use of coherent light scattering to study aggregation processes of macromolecules with special emphasis on the investigation of crystallization mechanisms of proteins.