SPECTROSCOPIC INVESTIGATIONS OF BIOLOGICAL SYSTEMS

生物系统的光谱研究

• 批准号: 3268436
• 负责人: GEORGE FEHER
• 金额: $ 24.2万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-05-10 至 1986-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3268436
• 关键词: analytical chemistry; electron transport; spectrometry

In the broadest sense, our research objectives are to understand biological processes and phenomena in terms of physical mechanisms and to explore the adaptation of physical phenomena and techniques to problems in biology. The more specific goals and problems to which we are addressing ourselves in this proposal are the following: 1. The mechanisms of energy conversion and electron transfer in a protein-chlorophyll complex solubilized from a bacterial membrane and the characterization of the protein complex. Different physical techniques (e.g., EPR, optical techniques, electron microscopy, Mossbauer spectroscopy, etc.) and biochemical methods (e.g., immunological, amino acid composition and sequence determination, gel filtration, gel electrophoresis, etc.) are being used. 2. The application of the technique of fluctuation spectroscopy to biology. In this technique the fluctuations (noise) are used to obtain information about the kinetic parameters of a system (i.e., rate constants) or from the number fluctuations, molecular weights of macromolecules (e.g., DNA) can be determined. 3. The use of coherent light scattering to study aggregation processes of macromolecules with special emphasis on the investigation of crystallization mechanisms of proteins.

从最广泛的意义上说，我们的研究目标是了解生物 过程和现象的物理机制，并探讨 使物理现象和技术适应生物学问题。 我们正在处理的更具体的目标和问题， 建议如下：1. 能量转换机制和 细菌溶解蛋白质叶绿素复合物中的电子转移 膜和蛋白质复合物的表征。 不同的物理 技术（例如，电子顺磁共振，光学技术，电子显微镜，穆斯堡尔 光谱学等）和生物化学方法（例如，免疫的、氨基酸 组成和序列测定，凝胶过滤，凝胶电泳， 等等）。被利用了 2. 波动技术的应用 光谱学到生物学 在这种技术中，波动（噪声）被用来 获得关于系统的动力学参数的信息（即，率 常数）或从数量波动，大分子的分子量 (e.g., DNA）可以确定。 3. 相干光散射的使用， 研究大分子的聚集过程，特别强调 蛋白质结晶机理的研究。