PRESSURE DISSOCIATION OF OLIGOMERIC PROTEINS & VIRUSES

低聚蛋白的压力解离

• 批准号: 3268243
• 负责人: GREGORIO WEBER
• 金额: $ 32.76万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 1993-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3268243
• 关键词: adenosinetriphosphatase; chemical aggregate; chemical association; chemical binding; conformation; fluorescence microscopy; fluorescence polarization; fluorescence spectrometry; fluorescent dye /probe; hydrostatic pressure; ligands; lysozyme; macromolecule; membrane lipids; membrane structure; molecular dynamics; protein structure; proteins; solutions; trypsin inhibitors; virus; virus morphology

The proposed research will concern the effects of high hydrostatic pressures, in the range of 1 to 3,000 atmospheres, upon macromolecular aggregates in solution. On one hand, we shall carry out a more thorough examination of the effects of pressure upon simple oligomeric proteins stressing the kinetics of the approach to equilibrium. The aim here will be to extend and better quantitate the observations of the conformational drift of separated subunits, the discovery of which has been the main outcome of our research in this subject for the past six years. On the other hand we will extend the study of the effects of pressure to the icosahedral viruses. Preliminary studies indicate that dissociation under pressure and reassociation upon decompression take place with appearance of evidently modified virus capsids. The differences between the native viruses and the species recovered after decompression will be examined as regards the role of the conformational drift in the formation of the modified aggregates. Their infectivity and immunogenic properties will be studied in detail in relation to the possibility of obtaining materials suitable for vaccination. The title of past grants (GM11223 1-24) reflected the development of fluorescence methodology. In the coming period we shall continue to use fluorescence techniques which have already proven indispensable to the present purpose, but I have changed the title to indicate our preoccupation with a well defined objective.

拟议的研究将涉及高静水压力的影响， 压力，在1至3，000个大气压的范围内， 溶液中的大分子聚集体。 一方面，我们将 对压力的影响进行更彻底的检查 在简单的寡聚蛋白质强调的动力学， 接近平衡。 这里的目标将是扩大和 更好地定量观察到的构象漂移 分离的亚基，它的发现一直是主要的 这是我们过去六年来在这方面的研究成果。 对 另一方面，我们将扩展压力效应的研究 二十面体病毒 初步研究表明， 压力下解离和减压下再结合 出现明显改变的病毒衣壳。 本土病毒与物种之间的差异 减压后恢复将检查关于 构象漂移在形成修饰的 集料. 它们的传染性和免疫原性将是 详细研究了获得 适合接种疫苗的材料。 过去赠款的标题（GM 11223 1-24）反映了 荧光方法学的发展。 今后一个时期 我们将继续使用荧光技术， 已经证明是不可或缺的目前的目的，但我有 改变了标题，以表明我们对一口井的关注， 明确的目标。