FLUORESCENCE OF BIOMOLECULES IN SOLUTION AND IN THE CELL

总结了近五年来的研究成果。它包括：1. 蛋白质动力学研究。2.压力对模型的影响 体系，单体和寡聚蛋白，脂质相变和 酶活性。3.多组分荧光体系的分辨率 相位和调制测量。4.不对称配基结合理论 以及它对血红蛋白的影响。5.朗之万案的应用 分布到极性荧光团-溶剂相互作用。6.合成和 使用新的环境敏感的荧光探针。一项计划 提出了未来五年的研究：调查 蛋白质动力学将被用于超快蛋白质的检测 用分子动力学预测酪氨酸运动及其对蛋白质的影响 蛋白质化学势的动力学。高压研究将会 继续以寡聚蛋白的解离为中心。一种新的 研究的主题将是压力对质子解离的影响 在基态和激发态的脂泡中的荧光团。 环境敏感的荧光探针将被用来研究 蛋白质在高稀释度下对温度和压力的稳定性。这个 适合于诱导单抗的这类探针的合成 将会被尝试。提出了一项全新的研究进展： 差示分析方法在荧光分析中的应用 用荧光显微镜研究细胞的溶液光谱。

The research of the last five years is summarized. It comprises: 1. Investigations of protein dynamics. 2. The effect of pressure upon model systems, monomeric and oligomeric proteins, lipid phase transitions and enzyme activity. 3. Resolution of multicomponent fluorescent systems by phase and modulation measurements. 4. Theory of asymmetric ligand binding and its implications for hemoglobin. 5. Application of the Langevin distribution to polar fluorophore-solvent interactions. 6. Synthesis and usage of new environmentally sensitive fluorescent probes. A plan of research for the coming five year period is presented: Investigations on protein dynamics will be directed to the detection of the ultrafast protein tyrosine motions predicted by molecular dynamics and to the influence of dynamics in the chemical potential of proteins. High-pressure studies will be continued centering on the dissociation of oligomeric proteins. A new subject of study will be the influence of pressure upon proton dissociation of fluorophores in lipid vesicles, in both ground and excited state. Environmentally sensitive fluorescent probes will be employed to study the stability of proteins to temperature and pressure at high dilution. The synthesis of probes of this type suitable to elicit monoclonal antibodies will be attempted. One completely new research development is proposed: The application of differential methods current in the fluorescence spectroscopy of solutions to the study of cells by fluorescence microscopy..