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FLUORESCENCE OF BIOMOLECULES IN SOLUTION AND IN THE CELL

溶液和细胞中生物分子的荧光

基本信息

批准号：
3268246
负责人：
GREGORIO WEBER
金额：
$ 14.79万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
起止时间：
1978-05-01 至 1988-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3268246
关键词：
adenosinetriphosphatase cells chemical binding conformation fluorescence microscopy fluorescence polarization fluorescence spectrometry fluorescent dye /probe ligands lysozyme mechanical pressure membrane lipids membrane structure molecular energy level solutions thermodynamics trypsin inhibitors

项目摘要

The research of the last five years is summarized. It comprises: 1. Investigations of protein dynamics. 2. The effect of pressure upon model systems, monomeric and oligomeric proteins, lipid phase transitions and enzyme activity. 3. Resolution of multicomponent fluorescent systems by phase and modulation measurements. 4. Theory of asymmetric ligand binding and its implications for hemoglobin. 5. Application of the Langevin distribution to polar fluorophore-solvent interactions. 6. Synthesis and usage of new environmentally sensitive fluorescent probes. A plan of research for the coming five year period is presented: Investigations on protein dynamics will be directed to the detection of the ultrafast protein tyrosine motions predicted by molecular dynamics and to the influence of dynamics in the chemical potential of proteins. High-pressure studies will be continued centering on the dissociation of oligomeric proteins. A new subject of study will be the influence of pressure upon proton dissociation of fluorophores in lipid vesicles, in both ground and excited state. Environmentally sensitive fluorescent probes will be employed to study the stability of proteins to temperature and pressure at high dilution. The synthesis of probes of this type suitable to elicit monoclonal antibodies will be attempted. One completely new research development is proposed: The application of differential methods current in the fluorescence spectroscopy of solutions to the study of cells by fluorescence microscopy..

总结了近五年来的研究成果。它包括：1. 蛋白质动力学研究。 2. 压力对模型的影响系统，单体和寡聚体蛋白质，脂质相变和酶活性 3. 多组分荧光体系的分离相位和调制测量。 4.不对称配体结合理论以及它对血红蛋白的影响。 5. Langevin的应用分布到极性荧光团-溶剂相互作用。 6. 合成与使用新的环境敏感的荧光探针。计划的未来五年的研究：调查蛋白质动力学将直接用于检测超快蛋白质酪氨酸运动预测的分子动力学和影响，蛋白质化学势的动力学。高压研究将继续集中在寡聚蛋白质的解离。一个新研究的主题是压力对质子离解的影响的脂质囊泡中的荧光团，在基态和激发态。环境敏感的荧光探针将被用来研究蛋白质在高稀释度下对温度和压力的稳定性。的适合于引发单克隆抗体的这类探针的合成将尝试。提出了一项全新的研究进展：微分电流法在荧光分析中的应用光谱的解决方案，以研究细胞的荧光显微镜。