CELLULAR MECHANISMS OF ACQUIRED COLOR VISION LOSS

此应用程序的长期目标是调查 视杆细胞和视锥细胞之间的组织化学差异及其利用 不同之处，以明确人类体内发生的退行性变化 视网膜感光细胞与衰老和疾病的关系。 先前的研究揭示了组织化学和形态上的差异 在蓝色敏感的锥体和红色和绿色敏感的锥体之间 将会被扩展。将获得进一步的文档，其中包含 球果的亚群，通过其缺乏碳酸酐酶来识别 (CA)酶活性，是对蓝色敏感的锥体。具体地说，一个 将尝试将CA的免疫细胞化学与酶相关联 人类视网膜中的组织化学。现有的推定抗体 红色/绿色圆锥体和蓝色圆锥体将与CA分布进行比较。蓝色 锥体也将通过接触重要染料和强烈的染料来鉴定。 光谱光。标记或损坏的视锥将与CA进行比较 分发。正常解剖结构的形态定量 与红色和绿色锥体相比，蓝色锥体的特征将是 在光镜(LM)和电子显微镜(EM)水平进行。 将CA的超微结构定位用于EM研究。 视网膜疾病中获得性色觉障碍的发病机制将是 通过检查蓝色的形态和流行率的变化进行了研究 几种疾病中锥体种群与红色/绿色锥体种群的比较 如视网膜脱离、糖尿病视网膜病变和青光眼。外科手术 标本和动物模型将被用于视网膜 超然。具有代表性糖尿病视网膜病变分期的供眼 将会被检查。青光眼将用人类供体材料和 高眼压动物模型的建立。所获得的关于时间进程的知识 退行性变化、损伤的可逆性和相对性 四种类型光感受器的易感性可能会影响接受性 治疗方法。

This application's long-term objectives are to investigate the histochemical differences between rods and cones and, exploiting these differences, to specify the degenerative changes that occur in human retinal photoreceptor cells with aging and disease. Previous studies revealing histochemical and morphological differences between the blue-sensitive cones and the red- and green sensitive cones will be expanded. Further documentation will be obtained that a sub-population of cones, identified by its absence of carbonic anhydrase (CA) enzymatic activity, is the blue-sensitive cones. Specifically, an attempt will be made to correlate immunocytochemistry for CA with enzyme histochemistry in the human retina. Existing antibodies to putative red/green cones and to blue cones will be compared to CA distribution. Blue cones will also be identified by exposure to vital dyes and to strong spectral lights. The labeled or damaged cones will be compared to CA distribution. Morphological quantitation of the normal anatomical characteristics of blue cones compared to red and green cones will be performed at both the light (LM) and electron microscopic (EM) level. Ultrastructural localization of CA will be employed for the EM studies. The pathogenesis of acquired dyschromatopsia in retinal disease will be studied by examining changes in the morphology and prevalence of the blue cone population versus the red/green cone population in several disorders such as retinal detachment, diabetic retinopathy and glaucoma. Surgical specimens as well as an animal model will be utilized for retinal detachment. Donated eyes with representative stages of diabetic retinopathy will be examined. Glaucoma will be studied with human donor material and an animal model of ocular hypertension. Knowledge gained of the time course of degenerative changes, reversibility of damage and comparative susceptibilities of the four types of photoreceptors may influence accepted approaches to treatment.