ADVANCED ELECTRON MICROSCOPICS OF CRYSTALLINE LENS

晶状体的先进电子显微镜

• 批准号: 3263149
• 负责人: Jer Kuszak
• 金额: $ 0.91万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3263149
• 关键词: Anura; Pongidae; aging; baboons; cataract; cell fusion; cell membrane; cellular pathology; cytoskeleton; electron microscopy; fresh water environment; growth /development; histopathology; human tissue; intercellular connection; laboratory rat; lens proteins; lyophilization; proteins; scanning electron microscopy; stainings

The long term objective is to gain a comprehensive qualitative and quantitative knowledge of primate (monkey, baboon and human) and lower vertebrate lens morphology. Specifically, advanced electron microscopic techniques (stereo SEI-SEM and BEI-SEM, stereo BEI-TEM and HVEM and freeze-etch replication will be used to assess various parameters of vertebrate lens fiber cell morphology as a function of topography and age. Correlative stereo SEI-SEM, BEI-SEM, BEI-TEM and light microscopy will be used to examine; 1. the surface and internal morphology, shape, size, and distribution of interdigitations, microvilli and ridges of lens fiber cells; 2. the total membrane surface area of lens fiber cells in growth rings as a function of age will be determined from spheroidal geometry formulas utilizing measurements of fiber thickness, lens equatorial diameter and lens antero-posterior diameter and incorporating the additional amount of membrane surface area contributed by interdigitations, microvilli and ridges; 3. the alteration of primate lens suture morphology from y suture to star suture will be examined as a function of growth ring age. From these findings, computer reconstructions of primate lenses will be made to determine the age related alterations in the structural pattern formed along the primate lens polar axis through which light rays are transmitted; and 4. the contribution of cell-to-cell fusion to the ordered structure of primate lenses. Correlative BEI-SEM and SEI-SEM and STEM and BEI-TEM will be utilizing selective staining of specific lens fiber cell components will be used to qualitatively examine; 1. the interlocking pattern between microvilli and ridges on primate lengs fiber cells; 2. localization and identification of the internal cytoskeletal components of lens fiber cell interdigitations; and 3. localization of fusion zones and identification of their internal cytoskeletal components. Freeze-etch replication will be used to determine the following parameters of lens fiber celll morphology; 1. the morphology, size, number and distribution of communicating junctions; 2. the morphology, size and number of communicating junctional IMPs; 3. the morphology, size and number of non-junctional IMPs. The importance of such studies is derived from the fact that alterations in lens morphology have been noted in all types of cataract. These studies will aid in establishing the norms for future comparative studies of lens morphology alterations heralded by changes in lens biochemistry and physiology during cataractogenesis.

长期目标是获得全面的质量和 灵长类动物（猴子、狒狒和人类）和低等动物的定量知识 脊椎动物透镜形态学。 特别是先进的电子显微镜 技术（立体SEI-SEM和BEI-SEM，立体BEI-TEM和HVEM， 冷冻蚀刻复制将用于评估各种参数 脊椎动物透镜纤维细胞形态作为地形和年龄的函数。 相关的立体SEI-SEM、BEI-SEM、BEI-TEM和光学显微镜将在 用于检查; 1。表面和内部形态、形状、尺寸和 透镜纤维的交错、微绒毛和脊的分布 细胞; 2.生长中的透镜纤维细胞的总膜表面积 年轮作为年龄的函数将从椭球体几何学中确定 利用纤维厚度、透镜赤道测量的公式 直径和透镜前后直径，并结合 由交错作用贡献的额外量的膜表面积， 微绒毛和脊; 3.灵长类动物透镜缝形态改变 从y缝线到星星缝线的变化将作为年轮的函数进行检查 年龄 根据这些发现，灵长类动物晶状体的计算机重建将 以确定结构模式中与年龄相关的变化 它沿着灵长类动物的透镜极轴形成，光线通过该极轴 传输;和4。细胞间融合对有序细胞的贡献 灵长类动物晶状体的结构 相关BEI-SEM和SEI-SEM和STEM以及 BEI-TEM将利用特定透镜纤维细胞的选择性染色 成分将用于定性检查; 1.互锁 微绒毛和脊之间的模式在灵长类动物的毛纤维细胞; 2. 的内部细胞骨架成分的定位和鉴定 透镜纤维细胞交错;和3.融合区的定位， 鉴定其内部细胞骨架成分。 冷冻蚀刻 复制将用于确定透镜的以下参数 纤维细胞形态; 1.形态、大小、数量和分布 交通枢纽; 2.形态、大小和数量 通信连接IMP; 3.形态、大小和数量 非交界性IMP。 这些研究的重要性来自于这样一个事实，即 透镜形态在所有类型的白内障中都有发现。 这些研究 将有助于建立规范，为未来的比较研究透镜 由透镜生物化学变化预示的形态学改变， 白内障形成过程中的生理学。