ADVANCED ELECTORN MICROSCOPIC STUDIES OF CRYSTALLINE LEN

晶体 LEN 的高级电子显微镜研究

• 批准号: 3263151
• 负责人: Jer Kuszak
• 金额: $ 8.71万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3263151
• 关键词: aging; cataract; cell fusion; cell membrane; cellular pathology; cytoskeleton; electron microscopy; fresh water environment; growth /development; histopathology; human tissue; intercellular connection; lens proteins; lyophilization; scanning electron microscopy; stainings

The long term objective is to gain a comprehensive qualitative and quantitative knowledge of primate (monkey, baboon and human) and lower vertebrate lens morphology. Specifically, advanced electron microscopic techniques (stereo SEI-SEM and BEI-SEM, stereo BEI-TEM and HVEM and freeze-etch replication will be used to assess various parameters of vertebrate lens fiber cell morphology as a function of topography and age. Correlative stereo SEI-SEM, BEI-SEM, BEI-TEM and light microscopy will be used to examine; 1. the surface and internal morphology, shape, size, and distribution of interdigitations, microvilli and ridges of lens fiber cells; 2. the total membrane surface area of lens fiber cells in growth rings as a function of age will be determined from spheroidal geometry formulas utilizing measurements of fiber thickness, lens equatorial diameter and lens antero-posterior diameter and incorporating the additional amount of membrane surface area contributed by interdigitations, microvilli and ridges; 3. the alteration of primate lens suture morphology from y suture to star suture will be examined as a function of growth ring age. From these findings, computer reconstructions of primate lenses will be made to determine the age related alterations in the structural pattern formed along the primate lens polar axis through which light rays are transmitted; and 4. the contribution of cell-to-cell fusion to the ordered structure of primate lenses. Correlative BEI-SEM and SEI-SEM and STEM and BEI-TEM will be utilizing selective staining of specific lens fiber cell components will be used to qualitatively examine; 1. the interlocking pattern between microvilli and ridges on primate lengs fiber cells; 2. localization and identification of the internal cytoskeletal components of lens fiber cell interdigitations; and 3. localization of fusion zones and identification of their internal cytoskeletal components. Freeze-etch replication will be used to determine the following parameters of lens fiber celll morphology; 1. the morphology, size, number and distribution of communicating junctions; 2. the morphology, size and number of communicating junctional IMPs; 3. the morphology, size and number of non-junctional IMPs. The importance of such studies is derived from the fact that alterations in lens morphology have been noted in all types of cataract. These studies will aid in establishing the norms for future comparative studies of lens morphology alterations heralded by changes in lens biochemistry and physiology during cataractogenesis.

我们的长期目标是获得全面的定性和 灵长类(猴子、狒狒和人类)及以下动物的定量知识 脊椎动物晶状体形态。具体地说，高级电子显微镜 技术(立体声SEI-SEM和BEI-SEM，立体声BEI-TEM和HVEM和 冷冻蚀刻复制将被用来评估各种参数 脊椎动物晶状体纤维细胞形态与地形和年龄的关系。 相关的立体SEI-SEM、BEI-SEM、BEI-TEM和光学显微镜 用于检验；1.表面和内部的形态、形状、大小和 晶状体纤维的交错、微绒毛和脊状分布 生长中的晶状体纤维细胞的膜表面积。 年轮作为年龄的函数将由球体几何确定 利用光纤厚度、透镜赤道测量的公式 直径和镜片前后径，并结合 交错指所贡献的额外的膜表面积， 3.灵长类晶状体缝线形态的改变 从Y形缝合到星形缝合将作为生长环的函数进行检查。 年龄。根据这些发现，灵长类晶状体的计算机重建将 以确定结构模式中与年龄相关的变化 沿着灵长类晶状体的极轴形成，光线通过它 传输；4.细胞间融合对有序的贡献 灵长类晶状体的结构。BEI-SEM和SEI-SEM与STEM的相关性 BEI-TEM将利用特定晶状体纤维细胞的选择性染色 将使用部件进行定性检查；1.联锁 灵长类Lengs纤维细胞上的微绒毛和脊之间的模式； 猪瘟患者体内细胞骨架成分的定位与鉴定 晶状体纤维细胞交错；3.融合区和 它们的内部细胞骨架成分的鉴定。冷冻蚀刻 复制将用于确定透镜的以下参数 纤维细胞形态：1.纤维细胞的形态、大小、数量和分布 2.交汇点的形态、大小和数量 3.交界性IMP的形态、大小和数量 非结合型小鬼。 这类研究的重要性源于这样一个事实，即 晶状体形态在所有类型的白内障中都有发现。这些研究 将有助于为今后的镜片比较研究建立规范 晶状体生化和组织化学改变预示的形态改变 白内障发生中的生理学。