FLAME - Fret Light-sheet Automated Micro Extensometer

FLAME - 品丝光片自动微型引伸计

• 批准号: BB/T01167X/1
• 负责人: Sarah Robinson
• 金额: $ 12.94万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT01167X%2F1
• 关键词: FLAME; Fret; Light; sheet; Automated

Understanding how plants obtain their size and shape is vital as it controls the functions of organs, their ability to withstand stress and regulates yields. Plant cells are surrounded by cell walls. In order for the cells to grow these walls must soften so that they can expand. This softening is coordinated so that parts of the tissue soften and others do not, enabling the emergence of complex shapes. Where this cell wall softening occurs is controlled by gene networks and the actions of the hormones that they encode. There is also feedback that exists within the system whereby the gene expression can be controlled by mechanical stresses that build up as a result of their actions. The direct link between gene expression and the final shape of the organ is poorly understood due to the difficulties associated with obtaining data that show gene expression or hormone levels at the same time are revealing mechanical properties. Most current methods require the sequential acquisition of data or are restricted to the surface of the tissue and thus do not provide information on the internal layers. Here we propose to address this by combining a small extensometer that can apply forces to tissue with a light-sheet microscope that is capable of imaging internal layers rapidly and with high resolution. This will provide insight into the process of development and also other processes that feed into it.

了解植物如何获得其大小和形状至关重要，因为它控制器官的功能、抵抗压力和调节产量的能力。植物细胞被细胞壁包围。为了让细胞生长，这些细胞壁必须软化，以便它们能够扩张。这种软化是协调一致的，因此组织的一部分软化而其他组织则不软化，从而能够出现复杂的形状。细胞壁软化发生的位置是由基因网络及其编码的激素的作用控制的。系统内存在反馈，基因表达可以通过其行为产生的机械应力来控制。由于获取显示基因表达或激素水平同时揭示机械特性的数据存在困难，人们对基因表达与器官最终形状之间的直接联系知之甚少。大多数当前方法需要顺序采集数据或仅限于组织表面，因此不提供有关内层的信息。在这里，我们建议通过将可以向组织施加力的小型引伸计与能够快速且高分辨率地对内层成像的光片显微镜相结合来解决这个问题。这将提供对开发过程以及参与其中的其他过程的深入了解。