METABOLISM OF OCULAR SURFACE EPITHELIUM

眼表上皮的代谢

• 批准号: 3262249
• 负责人: RICHARD A THOFT
• 金额: $ 22.48万
• 依托单位: EYE AND EAR HOSPITAL OF PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 1987-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3262249
• 关键词: cornea disorder; corneal epithelium; disease /disorder model; electron microscopy; eye disorder; histochemistry /cytochemistry; human tissue; immunochemistry; keratoplasty; membrane activity; organ culture; stress

Ocular surface diseases, affecting the corneal and conjunctival epithelia and immediately subjacent tissues, are among the most difficult external eye diseases to treat. The overall goals of this grant are to improve our understanding of the normal and diseased conjunctival and corneal epithelia (the ocular surface epithelium) with the eventual aim being the ability to define and manipulate some of the factors responsible for ocular surface disease. The first specific aim of the grant is to study the healing, replication, mucin production, and goblet cell status of the conjunctiva in normal and diseased eyes. This will be done using rabbit and human tissue. Healing will be studied in vivo by planimetry, and by histology. Replication, mucin production, and goblet cell status will be studied in conjunctival biopsies using organ culture and histological techniques. The second major aim is to study the acute transformation of conjunctival to corneal epithelium by evaluating the completeness of transformation as a function of time in rabbits, and by determining whether a similar process of transformation occurs in rats. Third, we will study the role of conjunctiva in the normal maintenance of corneal epithelium by evaluating clinically and histologically the effect of conjunctival injury on the corneal epithelium in vivo in rabbits. Fourth, we will continue studies of the metabolism of the corneal epithelium in response to stress. Finally, special attention will be directed to studies of persistent epithelial defect, a serious complication of many ocular surface disorders. The nature of the cell and substrate surfaces will be analysed immunohistochemically in tissues from patients with epithelial defects, and the results of those studies will be correlated with the clinical characteristics of the defects. Also, we will attempt to develop an animal model of persistent epithelial defect by combining a traumatic with a metabolic insult. By such studies, we hope to be able to define and manipulate some of the factors responsible for ocular surface disease.

影响角膜和结膜上皮的眼表疾病 和直接的皮下组织，是最困难的外部 这项拨款的总体目标是改善我们的 了解正常和患病的结膜和角膜上皮 (the眼表面上皮），最终目的是能够 定义和操纵一些影响眼表的因素 疾病 赠款的第一个具体目标是研究治疗， 复制，粘蛋白的生产，和杯状细胞状态的结膜， 正常和患病的眼睛。 这将使用兔子和人来完成 组织. 愈合将通过面积测量法和组织学在体内研究。 复制，粘蛋白的产生，和杯状细胞的状态将在 使用器官培养和组织学技术进行结膜活检。 的 第二个主要目的是研究结膜炎急性转化为 角膜上皮通过评估转化的完整性作为 在兔子的时间函数，并通过确定是否类似的过程， 在老鼠身上发生了转变。 第三，我们会研究 结膜在正常维持角膜上皮的评价 从临床和组织学上观察了结膜损伤对 兔角膜上皮的活体研究。 第四，继续研究 角膜上皮对压力的反应。 最后， 将特别关注持续性上皮细胞的研究， 缺陷，许多眼表疾病的严重并发症。 的 将分析电池和基板表面的性质 在上皮缺陷患者的组织中进行化学计量， 这些研究的结果将与临床 缺陷的特征。 同时，我们将尝试开发一种动物 持续性上皮缺损的模型，通过结合创伤性 代谢损伤 通过这样的研究，我们希望能够定义和 操纵一些导致眼表疾病的因素。