ENZYME MECHANISMS AND ENZYME INHIBITORS

S-腺苷同型半胱氨酸酶。2‘-DAD对SAHase失活机制的研究 被调查。将合成2‘~3H-DAD，以确定~3H 在失活过程中被释放。统一标记的C14 2-d‘Ad也将 合成以确定2‘DAD糖部分的命运 失活。 B12-辅酶。合成了C-5‘手性标记B12辅酶。 将进行实验以确定在以下情况下是否发生外消旋 在厌氧条件下，辅酶与酶结合。这将是 由核磁共振波谱学完成。 1-P，5-S-甲基核糖水解酶。该酶将被分离出来，其机制是 审查了行动。1-P，5-S-Me-核糖是由S-Me-腺苷衍生而来。这种酶 将被分离纯化，并对其反应机理进行了研究。 最初要回答的问题是：氧气是必需的吗？这种酶是否含有 不是吗？

S-Adenosylhomocysteinase. The mechanism of inactivation of SAHase by 2'dAd will be investigated. 2'3H dAd will be synthesized in order to determine whether 3H is released during inactivation. Uniformly labeled C14 2-d'Ad will also be synthesized to determine the fate of the sugar moiety of 2'dAd subsequent to inactivation. B12-Coenzyme. C-5' chirally labeled B12-coenzyme has been synthesized. Experiments will be carried out to determine whether racemization occurs when the coenzyme is bound to the enzyme under anaerobic conditions. This will be done by NMR spectroscopy. 1-P, 5-S-Me-ribose hydrolase. This enzyme will be isolated and its mechanism of action examined. 1-P, 5-S-Me-ribose is derived from S-Me-adenosine. The enzyme will be isolated and purified and its reaction mechanism will be examined. Initial questions to be answered are: Is O2 required? Does the enzyme contain NAD?