ENZYME MECHANISMS AND ENZYME INHIBITORS

S-腺苷高半胱氨酸酶。 2 'dAd对SAHase的失活机制将进一步阐明 追究 将合成2 '3 H dAd，以确定3 H dAd是否 在灭活过程中释放。 均匀标记的C14 2-d 'Ad也将被 合成，以确定2 'dAd的糖部分的命运， 失活 B12-辅酶。 合成了C-5'手性标记的B12-辅酶。 将进行实验以确定在以下情况下是否发生外消旋化： 辅酶在厌氧条件下与酶结合。 这将是 通过NMR光谱学。 1-P，5-S-Me-核糖水解酶。 这种酶将被分离出来，其机制， 检查行动。 1-β，5-S-Me-核糖衍生自S-Me-腺苷。 酶 分离纯化，并探讨其反应机理。 首先要回答的问题是：是否需要O2？ 这种酶是否含有 NAD？

S-Adenosylhomocysteinase. The mechanism of inactivation of SAHase by 2'dAd will be investigated. 2'3H dAd will be synthesized in order to determine whether 3H is released during inactivation. Uniformly labeled C14 2-d'Ad will also be synthesized to determine the fate of the sugar moiety of 2'dAd subsequent to inactivation. B12-Coenzyme. C-5' chirally labeled B12-coenzyme has been synthesized. Experiments will be carried out to determine whether racemization occurs when the coenzyme is bound to the enzyme under anaerobic conditions. This will be done by NMR spectroscopy. 1-P, 5-S-Me-ribose hydrolase. This enzyme will be isolated and its mechanism of action examined. 1-P, 5-S-Me-ribose is derived from S-Me-adenosine. The enzyme will be isolated and purified and its reaction mechanism will be examined. Initial questions to be answered are: Is O2 required? Does the enzyme contain NAD?