NEUROGENETICS OF DROSOPHILA REPRODUCTION

将使用一种组合来分析果蝇的繁殖 遗传、分子、行为和神经生物学方法。 对相关基因的操纵--在它们的正常和突变中 Forms--外加对表型影响的详细分析 这些因素主要是从以下角度考虑的： 这些双翅目昆虫控制着它们复杂的行动；但这些种类的 遗传导致的神经功能缺陷也与 在其他生物中具有更高大脑功能的神经遗传学。 具体地说，突变导致听觉异常 交流--刺耳、不和谐和胆碱-- 乙酰转移酶-TS--将在哪里以及如何进行研究 这些变体扰乱了中枢神经系统的结构或功能；关于 目的是分离和分析与之相对应的克隆序列 这些基因座(包括主要基因产物的位置 表示)。新的“翅膀振动”突变体将被筛选(在 在某些情况下，扩展已知基因座的变异数组，例如 CAC和DIS)。声学中的节奏元素 将进一步调查通信，主要是与 参考这些振荡的神经底物(例如， 受神经传导突变的影响)。嗅觉 繁殖中的交流将得到更深入的研究 关于接收和处理相关的 信息素，在外围进行生理研究，以及更多 对于一个突变体--嗅觉-D--来说，基本上 对这些化学感官信号没有反应。一无所获的人 突变，这(至少)导致异常产生 信息素，将被分析--包括分子方法 Tue Locus；这将允许询问有关位置和位置的问题 当该基因被表达时，提供了关于该病病因的线索 由果糖引起的生殖异常的“综合征”。 有条件的求爱--众所周知，这涉及到“加工” 听觉和嗅觉刺激--将被解剖 从遗传学上讲，主要是通过确定神经基础 三个特定的学习/记忆突变体在 男性/女性互动的这些依赖经验的方面； 将与新兴知识进行关联(来自其他 研究人员)关于相关基因的产物在哪里 在动物的中枢神经系统中表达。

Reproduction will be analyzed in Drosophila, using a combination of genetic, molecular, behavioral, and neurobiological approaches. Manipulation of the pertinent genes--in their normal and mutant forms--plus detailed analysis of the phenotypes influenced by these factors is considered primarily from the standpoint of how these diptera control their complex actions; but these kinds of genetically induced defects in neural function also are relevant to the neurogenetics of higher brain functions in other organisms. Specifically, mutations causing ABERRANT AUDITORY COMMUNICATION--cacophony, dissonance, and Choline- acetyltransferase-TS--will be studied, in terms of where and how these variants disrupt CNS structure or function; and with regard to isolation and analysis of cloned sequences corresponding to those loci (including where the primary gene products are expressed). New "wing vibration" mutants will be screened for (in some cases to expand the array of variants at known loci such as cac and diss). A RHYTHMIC ELEMENT of acoustic communication will be further investigated, primarily with reference to the neural substrates of these oscillations (e.g., as affected by nerve-conduction mutations). OLFACTORY COMMUNICATION in reproduction will be more deeply delved into, in regard to reception and processing of the relevant pheromones, studied physiologically at the periphery and more centrally with respect to a mutant--olfactory-D--that essentially fails to respond to these chemosensory cues. The fruitless mutation, which (at least) causes aberrant production of pheromones, will be analyzed--including molecular approaches to tue locus; this will allow questions to be asked about where and when the gene is expressed, to provide clues on the etiology of the "syndrome" of reproductive abnormalities induced by fru. CONDITIONED COURTSHIP--known to involve the "processing" of both auditory and olfactory stimuli--will be dissected genetically, mostly by determination of the neural substrates of three specific learning/memory mutants that are aberrant in these experience-dependent aspects of male/female interactions; correlations will be made with emerging knowledge (from other investigators) on where the products of the relevant genes are expressed in the animal's CNS.