NEUROGENETICS OF DROSOPHILA REPRODUCTION

果蝇的生殖神经遗传学涉及一个不断扩大的阵列 行为和神经突变体以及分子操作 涉及影响苍蝇特定性别特征的因素 神经系统。扩展对半经典求爱的分析 基因、钙通道多肽和钙通道多肽的作用 我们将对核酸结合蛋白进行剖析。对于前一位 这两个基因，杂音，实验包括分子- 求爱歌曲缺陷和视觉系统缺陷的遗传分析 (后者是由某些CAC基因突变引起的)。 转录后变异在频道质量中的意义 将进行评估，包括分子操作和行为 涉及S小学的疑似核糖核酸编辑事件的生物检测 产品。瞬时A基因也可能发生突变，从而导致 唱歌和视觉异常。诺纳空间的意义 我们将主要从小说的角度来分析表达方式 与转基因相关的求偶嗡嗡声异常 对正常等位基因的操纵。诺娜和推定的 它编码的可能与CAC相互作用的RNA结合蛋白将是 确定的，部分是通过确定在体内和 NONA基因工程突变影响转录后加工 钙离子通道RNA CAC的行为学和分子遗传学 暗示了求爱歌曲在进化上的一种分化方式： 钙引起的模式神经元输出的调制- 渠道变化；这将通过分析内部 CAC的特定亲缘关系及其分子变异的生物测定 转基因雄性。性别决定的遗传学将进一步 结合行为和信息素的求偶研究 分析无果和双性突变体；实验包括 求爱-嗡嗡异常的分子神经遗传学解剖 以及对特定性别的运动行为的分析。在某些情况下移动 新的方向，将分析女性接受性的遗传学 通过对一种新突变体的神经生物学和分子研究；另外， 可能的生物钟重置，由条件反射引起 求爱刺激，将被调查，看看是否可以相似 被哺乳动物在这一领域的一项新发现所吸引。-- 从表面上看，繁殖似乎具有广泛的意义。 这些现象的变异，由遗传在生物体中引起 从人类到人类，正在被越来越多地欣赏、分析， 并在实验中制造。有人认为神经发生学 和分子神经生物学的发现和原理，源于 部分关于果蝇求偶和交配的研究，可能 帮助设计和解释这一领域的调查 这将涉及到各种不同的生物体。

Reproductive neurogenetics in Drosophila involves a widening array of behavioral and neural mutants as well as molecular manipulations involving factors that influence sex-specific features of the fly's nervous system. To extend the analysis of semi-classical courtship genes, the roles played by calcium-channel polypeptides and nucleic-acid binding proteins will be dissected. For the former of these two genes, cacophony, the experiments include molecular- genetic analysis of courtship-song defects and visual-system ones as well (the latter being caused by certain of the cac-locus mutations). The significance of posttranscriptional variation in channel quality will be assessed, including molecular manipulations and behavioral bioassays of putative RNA editing events involving cac s primary product. The no-on-transient-A gene also can be mutated to cause singing and visual abnormalities. The significance of nonA's spatial expression will be dissected, principally in terms of a novel abnormality of courtship-humming sounds associated with transgene manipulations of the normal allele. How nonA and the putative RNA-binding protein it encodes may interact with cac will be determined, in part by determining whether in vivo an and engineered nonA mutations affect post-transcriptional processing of the Ca2+-channel RNA cac's behavioral and molecular-genetics suggests one way that courtship songs diverge evolutionarily: by modulations of patterned neuronal outputs caused by calcium- channel variations; this will be investigated by analyzing inter- specific relatives of cac and bioassays of the molecular variants in transgenic males. The genetics of sex determination will be further merged with courtship studies by behavioral and pheromonal analysis of fruitless and doublesex mutants; the experiments include molecular neurogenetic dissection of courtship-hum abnormalities and analysis of sex-specific locomotor behavior. To move in certain new directions, the genetics of female receptivity will be analyzed by neurobiological and molecular studies of a new mutant; also, possible resets of a biological clock, induced by conditioned courtship stimuli, will be investigated to see if parallels can be drawn to a novel finding in this area from mammals. -- Reproduction seems on the face of it to have broad significance. Variations of these phenomena, caused genetically in organisms ranging up to humans, are being increasingly appreciated, analyzed, and manufactured experimentally. It is suggested that neurogenetic and molecular neurobiological findings and principles, stemming in part from studies of Drosophila courtship and mating, may contribute to the design and interpretation of investigations in this area, which would involve a variety of different organisms.