NEUROGENETICS OF DROSOPHILA REPRODUCTION

果蝇繁殖的神经遗传学

基本信息

批准号：
6179876
负责人：
JEFFREY C HALL
金额：
$ 23.04万
依托单位：
BRANDEIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1977
资助国家：
美国
起止时间：
1977-12-01 至 2003-12-07
项目状态：
已结题

项目摘要

Reproductive neurogenetics in Drosophila involves a widening array of behavioral and neural mutants as well as molecular manipulations involving factors that influence sex-specific features of the fly's nervous system. To extend the analysis of semi-classical courtship genes, the roles played by calcium-channel polypeptides and nucleic-acid binding proteins will be dissected. For the former of these two genes, cacophony, the experiments include molecular- genetic analysis of courtship-song defects and visual-system ones as well (the latter being caused by certain of the cac-locus mutations). The significance of posttranscriptional variation in channel quality will be assessed, including molecular manipulations and behavioral bioassays of putative RNA editing events involving cac s primary product. The no-on-transient-A gene also can be mutated to cause singing and visual abnormalities. The significance of nonA's spatial expression will be dissected, principally in terms of a novel abnormality of courtship-humming sounds associated with transgene manipulations of the normal allele. How nonA and the putative RNA-binding protein it encodes may interact with cac will be determined, in part by determining whether in vivo an and engineered nonA mutations affect post-transcriptional processing of the Ca2+-channel RNA cac's behavioral and molecular-genetics suggests one way that courtship songs diverge evolutionarily: by modulations of patterned neuronal outputs caused by calcium- channel variations; this will be investigated by analyzing inter- specific relatives of cac and bioassays of the molecular variants in transgenic males. The genetics of sex determination will be further merged with courtship studies by behavioral and pheromonal analysis of fruitless and doublesex mutants; the experiments include molecular neurogenetic dissection of courtship-hum abnormalities and analysis of sex-specific locomotor behavior. To move in certain new directions, the genetics of female receptivity will be analyzed by neurobiological and molecular studies of a new mutant; also, possible resets of a biological clock, induced by conditioned courtship stimuli, will be investigated to see if parallels can be drawn to a novel finding in this area from mammals. -- Reproduction seems on the face of it to have broad significance. Variations of these phenomena, caused genetically in organisms ranging up to humans, are being increasingly appreciated, analyzed, and manufactured experimentally. It is suggested that neurogenetic and molecular neurobiological findings and principles, stemming in part from studies of Drosophila courtship and mating, may contribute to the design and interpretation of investigations in this area, which would involve a variety of different organisms.

果蝇中的生殖神经遗传学涉及扩大的阵列行为和神经突变体以及分子操纵涉及影响苍蝇的性别特定特征的因素神经系统。扩展对半古典求爱的分析基因，钙通道多肽和将解剖核酸结合蛋白。对于前者这两个基因，即cacophony，实验包括分子 - 对求爱的缺陷和视觉系统的遗传分析好（后者是由某些CAC-Locus突变引起的）。转录后通道质量变化的重要性将评估，包括分子操作和行为推定的RNA编辑事件的生物分析涉及CAC的主要产品。也可以将无频率A基因突变以引起唱歌和视觉异常。诺娜空间的意义表达将被解剖，主要是根据新颖的与Transgene相关的求爱的异常对正常等位基因的操纵。诺娜和推定的方式它编码的RNA结合蛋白可能与CAC相互作用确定部分通过确定体内AN和工程的非A突变会影响转录后处理 Ca2+ - 通道RNA CAC的行为和分子基因提出一种求爱歌曲进化上不同的一种方式：由钙引起的图案化神经元输出的调节通道变化；这将通过分析间 CAC的特定亲属和分子变体的生物测定转基因男性。性别决定的遗传学将进一步通过行为和门经来与求爱研究合并分析无结果和双塞克斯突变体；实验包括分子神经遗传学解剖求爱异常和性别特异性运动行为的分析。一定要移动新方向，将分析女性接受性的遗传学通过新突变体的神经生物学和分子研究；还，可能重置生物钟，由条件诱导将调查求爱刺激，以查看是否可以相似之处从哺乳动物中吸引了这一领域的新发现。 - - 繁殖似乎具有广泛的意义。这些现象的变化，在生物体上遗传引起越来越多地向人类，越来越受到赞赏，分析，并通过实验制造。建议神经遗传学和分子神经生物学发现和原理，一部分是从果蝇求爱和交配的研究中为此促进调查的设计和解释区域，将涉及各种不同的生物。