PHYSIOCHEMICAL STUDIES OF BIOCHEMICAL REACTIONS

这个项目的总体目标是应用物理化学 各种生物化学和生物化学的技术和原理 生物过程。尽管我们将继续努力，就像在 过去，要以决心和诠释为中心 在这类过程的热力学中，经常可以找到 得到了重要的非热力学结果。 预计在接下来的几年里，这项工作支持 这笔赠款将主要用于高 灵敏度差示扫描量热仪(DSC)联合研究 蛋白质、核酸和脂类。这项技术的威力 目前正在迅速扩张。 在蛋白质的情况下，主要的努力将是准确的 多个分子展开热力学的测定 多种蛋白质的突变形式，主要由 其他实验室的合作者。将包括溶菌酶 T4噬菌体、葡萄球菌核酸酶、酵母细胞色素c、 核糖核酸酶T1，T4基因32蛋白，E. Coli等。 关于DNA的B-Z转变的热力学，以及关于 最近的DSC实验表明DNA具有广泛的多态性， 将会继续。 差示扫描量热法是研究相变的一种很好的方法 我们将继续将DSC应用于纯净的 脂类及其与其他脂类、药物、蛋白质和 其他类型的添加溶质。 DSC工作将适当地通过等温平行进行 量热法和其他类型的测量。

The general aim in this project is to apply physicochemical techniques and principles to a wide variety of biochemical and biological processes. Although our efforts will continue, as in the past, to be centered on the determination and interpretation of the thermodynamics of such processes, it is frequently found that significant non-thermodynamic results are obtained. It is expected that during the next few years, the work supported by this grant will be concerned primarily with applications of high sensitivity differential scanning calorimetry (DSC) co the study of proteins, nucleic acids and lipids. The power of this technique is at present being rapidly expanded. In the case of proteins, major effort will go into accurate determinations of the thermodynamics of unfolding of numerous mutant forms of a wide variety of proteins, supplied mostly by collaborators in other laboratories. Included will be the lysozyme of T4 phage, staphylococcal nuclease, yeast cytochrome c, ribonuclease T1, gene 32 protein of T4, tryptophan repressor of E. coli, etc. Work on the thermodynamics of the B-Z transition of DNA, and on the extensive polymorphism of DNA indicated/by recent DSC experiments, will be continued. DSC is an excellent technique for studying the phase transitions of phospholipid bilayers, and we will continue to apply DSC to pure lipids and their mixtures with other lipids, drugs, proteins and other types of added solutes. The DSC work will be paralled as appropriate by isothermal calorimetry and other types of measurements.