PHYSIOCHEMICAL STUDIES OF BIOCHEMICAL REACTIONS

生化反应的物理化学研究

• 批准号: 3267858
• 负责人: JUDITH M STURTEVANT
• 金额: $ 14.57万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1974
• 资助国家: 美国
• 起止时间: 1974-10-15 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3267858
• 关键词: DNA; bacteriophage T4; binding proteins; calorimetry; conformation; cytochrome c; electron microscopy; enzyme structure; lysozyme; nuclease; nucleic acid structure; phosphoglycerate kinase; polynucleotides; protein structure; temperature; thermodynamics; transcription factor; virus protein; yeasts

The general aim in this project is to apply physicochemical techniques and principles to a wide variety of biochemical and biological processes. Although our efforts will continue, as in the past, to be centered on the determination and interpretation of the thermodynamics of such processes, it is frequently found that significant non-thermodynamic results are obtained. It is expected that during the next few years, the work supported by this grant will be concerned primarily with applications of high sensitivity differential scanning calorimetry (DSC) co the study of proteins, nucleic acids and lipids. The power of this technique is at present being rapidly expanded. In the case of proteins, major effort will go into accurate determinations of the thermodynamics of unfolding of numerous mutant forms of a wide variety of proteins, supplied mostly by collaborators in other laboratories. Included will be the lysozyme of T4 phage, staphylococcal nuclease, yeast cytochrome c, ribonuclease T1, gene 32 protein of T4, tryptophan repressor of E. coli, etc. Work on the thermodynamics of the B-Z transition of DNA, and on the extensive polymorphism of DNA indicated/by recent DSC experiments, will be continued. DSC is an excellent technique for studying the phase transitions of phospholipid bilayers, and we will continue to apply DSC to pure lipids and their mixtures with other lipids, drugs, proteins and other types of added solutes. The DSC work will be paralled as appropriate by isothermal calorimetry and other types of measurements.

本项目的总体目标是应用物理化学 技术和原理，以各种各样的生物化学和 生物过程。 尽管我们的努力将继续下去， 过去，以确定和解释为中心， 在这些过程的热力学中， 获得了重要的非热力学结果。 预计在未来几年内，这项工作将支持 这项补助金将主要涉及申请高 灵敏度差示扫描量热法（DSC）的研究 蛋白质、核酸和脂类的混合物。 这项技术的力量 目前正在迅速扩大。 在蛋白质的情况下，主要的努力将进入准确的 确定热力学展开的许多 各种蛋白质的突变形式，主要由 其他实验室的合作者。 包括溶菌酶 T4噬菌体、葡萄球菌核酸酶、酵母细胞色素c， 核糖核酸酶T1、T4的基因32蛋白、E. 大肠杆菌等。 研究DNA的B-Z跃迁的热力学， 最近的DSC实验表明DNA的广泛多态性， 将继续进行。 差示扫描量热法是研究相变的一种很好的方法 磷脂双层，我们将继续应用DSC纯 脂质及其与其他脂质、药物、蛋白质和 添加的其他类型的溶质。 DSC工作将酌情与等温 量热法和其他类型的测量。