CHARACTERIZATION OF MAMMALIAN MITOCHONDRIAL RIBOSOMES

哺乳动物线粒体含有不寻常的55S核糖体 组成和物理化学性质。整体而言 拟议研究的目的是阐明 哺乳动物线粒体核糖体的功能特性。 大多数研究将使用牛线粒体进行 核糖体--哺乳动物研究的模式系统 线粒体核糖体。对其功能的研究 线粒体核糖体将集中在 启动蛋白质合成。具体内容和要求 为了寻找不同的线粒体mRNA分子来 将研究有丝分裂体小亚基，核糖体 参与mRNA结合的蛋白质将通过 亲和探针的应用及其功能重构的研究 缺乏蛋白质的颗粒。 这些研究提供的庞大数据库确立了 牛线粒体核糖体作为一个综合模型系统 用于研究哺乳动物线粒体的核糖体。这些研究 它们阐明了分子组成和官能团 由哺乳动物线粒体上的活性中心组成 核糖体为解释有毒和有害的 抗菌药物和化疗药物的不良反应 线粒体核糖体的水平。

Mammalian mitochondria contain 55S ribosomes of unusual composition and physical-chemical properties. The overall objective of the proposed research is to elucidate the unique functional properties of mammalian mitochondrial ribosomes. Most of the studies will be carried out using bovine mitochondrial ribosomes, as a model system for studies of mammalian mitochondrial ribosomes. Studies on the function of mitochondrial ribosomes will center on the mechanisms of initiation of protein synthesis. The specificity and requirements for finding of different mitochondrial mRNA molecules to mitoribosomal small subunits will be studied, and ribosomal proteins involved in mRNA binding will be identified through the use of affinity probes and studies on the functional reconstitution of protein-deficient particles. The extensive data base provided by these studies established the bovine mitochondrial ribosome as a comprehensive model system for studying ribosomes of mammalian mitochondria. These studies which elucidate the molecular components and functional groups that comprise active centers on mammalian mitochondrial ribosomes provide the basis for interpreting the toxic and adverse side effects of antibiotics and chemotherapeutic agents at the level of the mitochondrial ribosome.