CHARACTERIZATION OF MAMMALIAN MITOCHONDRIAL RIBOSOMES

哺乳动物线粒体含有55S核糖体， 组成和物理化学性质。 整体 这项研究的目的是阐明 哺乳动物线粒体核糖体的功能特性。 大多数研究将使用牛线粒体进行 核糖体，作为哺乳动物研究的模型系统 线粒体核糖体 功能的研究 线粒体核糖体将集中在 蛋白质合成的起始。 特殊性和要求 寻找不同的线粒体mRNA分子， 将研究线粒体小亚基， 参与mRNA结合的蛋白质将通过 亲和探针的使用和功能重建的研究 缺乏蛋白质的颗粒。 这些研究提供的广泛数据库建立了 牛线粒体核糖体作为综合模型系统 用于研究哺乳动物线粒体的核糖体。 这些研究 其阐明了分子组分和官能团 由哺乳动物线粒体上的活性中心组成 核糖体提供了解释毒性和不良反应的基础， 抗生素和化疗药物的副作用 线粒体核糖体水平。

Mammalian mitochondria contain 55S ribosomes of unusual composition and physical-chemical properties. The overall objective of the proposed research is to elucidate the unique functional properties of mammalian mitochondrial ribosomes. Most of the studies will be carried out using bovine mitochondrial ribosomes, as a model system for studies of mammalian mitochondrial ribosomes. Studies on the function of mitochondrial ribosomes will center on the mechanisms of initiation of protein synthesis. The specificity and requirements for finding of different mitochondrial mRNA molecules to mitoribosomal small subunits will be studied, and ribosomal proteins involved in mRNA binding will be identified through the use of affinity probes and studies on the functional reconstitution of protein-deficient particles. The extensive data base provided by these studies established the bovine mitochondrial ribosome as a comprehensive model system for studying ribosomes of mammalian mitochondria. These studies which elucidate the molecular components and functional groups that comprise active centers on mammalian mitochondrial ribosomes provide the basis for interpreting the toxic and adverse side effects of antibiotics and chemotherapeutic agents at the level of the mitochondrial ribosome.