OPTICAL PROPERTIES OF BIOLOGICAL MACROMOLECULES

生物大分子的光学性质

• 批准号: 3271443
• 负责人: ROBERT W WOODY
• 金额: $ 14.69万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-12-01 至 1990-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3271443
• 关键词: Desulfovibrio; alcohol dehydrogenase; antibiotics; chemical condensation; chemical models; chromophore; circular dichroism; conformation; cyclic aminoacid; cyclic peptides; cytochrome c; cytochrome oxidase; flavoproteins; fluorescence; fluorescent dye /probe; hemoglobin; hemoprotein; histidine; human tissue; infrared microscopy; ion exchange chromatography; isozymes; lactate dehydrogenases; ligands; macromolecule; malate dehydrogenase; mathematics; metal complex; molecular weight; mutant; nuclear magnetic resonance spectroscopy; nucleotides; peptidases; polarimetry; synthetic peptide; tryptophan

The object of this project is to increase and extend the usefulness of circular dichroism to the study of macromolecular structure. During the coming year, efforts will be centered on the following areas: (a) polypeptide CD; (b) heme proteins; (c) dyes and nucleotides binding to dehydrogenases and kinases; and (d) actinomycin-DNA interactions. Further calculations on nonplanar peptides will be performed, as will studies of the twisted beta-sheets which are ubiquitous in proteins. For the latter, two models have recently been proposed, which will be used to provide idealized geometries. Real geometries taken from high resolution X-ray structures will also be considered. Experimental studies on the CD of myoglobin and monomeric hemoglobins reconstituted with modified porphyrins will be continued. Affinity chromatography with immobilized apomyoglobin will be used to try to resolve enantiomers of vanadyl porphyrins. Oriented films of cytochrome oxidase will continue to be studied with emphasis on obtaining improved quality in the films and extension to infrared studies. Studies of Ca ion 2-dependent ATPase in sarcoplasmic reticulum will also be initiated. The CD of tetraiodofluorescein and bromphenol blue bound to a series of kinases and dehydrogenases will be investigated. Ethenoadenine analogs of ATP and ADP will be utilized as well. Theoretical calculations on actinomycin-DNA interactions will be initiated. The CD of free actinomycin will first be treated theoretically. If these calculations are successful, studies of actinomycin complexes with dCpG and with DNA, following the model of Sobell and Jain, will be undertaken.

这个项目的目的是增加和扩大循环的有用性 二向色性用于大分子结构的研究。 在即将到来的一年里， 工作重点将集中在以下领域：（a）多肽CD;（B）血红素 （c）结合至酶和激酶的染料和核苷酸;以及 (d)放线菌素-DNA相互作用 非平面肽的进一步计算 将被执行，因为将研究扭曲的β-片层， 普遍存在于蛋白质中。 对于后者，最近提出了两种模型， 其将用于提供理想化的几何形状。 真实的几何体取自 还将考虑更高分辨率的X射线结构。 实验研究 在肌红蛋白和单体血红蛋白的CD上， 卟啉将继续。 固定化亲和层析 脱辅基肌红蛋白将用于尝试拆分氧钒卟啉的对映体。 细胞色素氧化酶的取向膜将继续得到重点研究 在获得改进的质量在电影和扩展到红外研究。 肌浆网钙离子依赖性ATP酶的研究也将在 启动。 四碘荧光素和溴酚蓝的CD结合到一系列 将研究激酶和磷酸酶。 乙烯腺嘌呤类似物 ATP和ADP也将被使用。 的理论计算 放线菌素-DNA相互作用将被启动。 游离放线菌素的CD将 首先要从理论上进行处理。 如果这些计算成功，研究 放线菌素与dCpG和DNA的复合物，遵循Sobell模型 和耆那教将被执行。