OPTICAL PROPERTIES OF BIOLOGICAL MACROMOLECULES

本项目的目的是增加和扩大通告的有用性 向大分子结构研究的二色性。在未来一年里， 工作将集中在以下领域：(A)多肽CD；(B)血红素 蛋白质；(C)与脱氢酶和激酶结合的染料和核苷酸；以及 (D)放线菌素与DNA的相互作用。关于非平面多肽的进一步计算 将进行，以及将对扭曲的β-折叠进行研究 普遍存在于蛋白质中。对于后者，最近提出了两种模型， 它将被用来提供理想化的几何图形。真实几何图形取自 高分辨率的X射线结构也将被考虑。实验研究 改性重组肌红蛋白和单体血红蛋白的CD研究 将继续使用卟啉。固定化亲和层析 去肌红蛋白将被用来尝试拆分钒基卟啉的对映体。 将继续重点研究细胞色素氧化酶的取向膜 关于提高胶片的质量和扩展到红外研究。 肌浆网钙离子依赖性ATPase的研究也将展开。 已启动。四碘荧光素和溴酚蓝的CD结合在一起 将对激活酶和脱氢酶进行研究。乙烯腺嘌呤类似物 ATP和ADP也将被利用。关于的理论计算 将启动放线菌素与DNA的相互作用。游离型放线菌素的CD 首先从理论上对待。如果这些计算是成功的，研究 放线菌素与dCpG和DNA的络合物，遵循Sobell模型 和Jain，都会被带走。

The object of this project is to increase and extend the usefulness of circular dichroism to the study of macromolecular structure. During the coming year, efforts will be centered on the following areas: (a) polypeptide CD; (b) heme proteins; (c) dyes and nucleotides binding to dehydrogenases and kinases; and (d) actinomycin-DNA interactions. Further calculations on nonplanar peptides will be performed, as will studies of the twisted beta-sheets which are ubiquitous in proteins. For the latter, two models have recently been proposed, which will be used to provide idealized geometries. Real geometries taken from high resolution X-ray structures will also be considered. Experimental studies on the CD of myoglobin and monomeric hemoglobins reconstituted with modified porphyrins will be continued. Affinity chromatography with immobilized apomyoglobin will be used to try to resolve enantiomers of vanadyl porphyrins. Oriented films of cytochrome oxidase will continue to be studied with emphasis on obtaining improved quality in the films and extension to infrared studies. Studies of Ca ion 2-dependent ATPase in sarcoplasmic reticulum will also be initiated. The CD of tetraiodofluorescein and bromphenol blue bound to a series of kinases and dehydrogenases will be investigated. Ethenoadenine analogs of ATP and ADP will be utilized as well. Theoretical calculations on actinomycin-DNA interactions will be initiated. The CD of free actinomycin will first be treated theoretically. If these calculations are successful, studies of actinomycin complexes with dCpG and with DNA, following the model of Sobell and Jain, will be undertaken.