FAST PHOTOSENSITIZED REACTIONS OF AROMATIC COMPOUNDS

在光疗过程中的目标是提供光 达到所需临床目的的组织剂量， 例如，在一个实施例中，在银屑病的“PUVA”疗法中，新生儿的光疗法 黄疸和恶性肿瘤的光动力疗法（PDT）。 组织是光学混浊介质，其中 辐射能量取决于光的散射和吸收 属性、尺寸和与外部介质的接口。 包埋在组织中的发色团对光的利用可以 用实验和分析方法进行研究， 组织光学 一种漫射光学分光光度计， 将使用积分球和光纤探针方法 测量，并将结果与 辐射传递的扩散近似 这些技术 将应用于体外系统，模拟生物物理学 和光化学阶段的PDT在分子水平上。 组织 由光散射脂质体组成并重新密封的模型 含有血卟啉的牛红细胞膜 衍生物（HPD）制剂和选择的替代PDT 敏化剂，将被用来研究波长的影响， 光敏剂浓度和光漂白对光动力学的影响 效率 要研究的HPD制剂包括 临床药物Photofrin II，富含活性的HPD 凝胶柱法测定“二血卟啉醚”组分 色谱法，99%DHE在a Sephadex LH-20上制备 列（由D博士提供。凯塞尔）和具有高酯的HPD 内容物通过在吡啶中用乙酸酐乙酰化制备。 脂质过氧化，鬼蛋白的交联，和 将采用外源酶探针的光灭活 进行损伤评估 类似的实验将在 掺入光敏剂的生物组织，包括马铃薯 石板和猪皮 该项目包括进一步发展 PDT治疗的分析光学剂量学模型 规划 这些模型将在地球物理水平上进行测试 与组织模型，并评价适用性， 与正在进行的PDT试验进行回顾性比较。

The objective in a phototherapy procedure is to deliver the light dose to the tissue that accomplishes the desired clinical objective, e.g., in "PUVA" therapy of psoriasis, light therapy of neonatal jaundice, and photodynamic therapy (PDT) of malignant tumors. Tissues are optically turbid media, in which the disposition of radiant energy depends on the light scattering and absorption properties, the dimensions, and interfaces with external media. The utilization of light by chromophores embedded in tissues can be investigated with the experimental and analytical methods of tissue optics. A diffuse optics spectrophotometer utilizing integrating spheres and optical fiber probe methods will be used for the measurements, and the results will be analyzed with the diffusion approximation for radiative transfer. These techniques will be applied to in vitro systems that model the photophysical and photochemical stages of PDT at the molecular level. Tissue phantoms consisting of light scattering liposomes and resealed beef erythrocyte membranes incorporating a hematoporphyrin derivative (HPD) preparation, and selected alternative PDT sensitizers, will be employed to study the effects of wavelengths, sensitizer concentration, and photobleaching on photodynamic efficiency. The HPD preparations to be investigated include the clinical drug Photofrin II, HPD enriched in the active "dihematoporphyrin ether" (DHE) constituent by gel column chromatography, 99% DHE prepared on an a Sephadex LH-20 column (provided by Dr. D. Kessel), and HPD with a high ester content prepared by acetylation with acetic anhydride in pyridine. Lipid peroxidation, cross-linking of ghost proteins, and photoinactivation of exogenous enzyme probes will be employed for damage evaluation. Similar experiments will be done with biological tissues incorporating photosensitizers, including potato slabs and pig skin. The project includes the further development of analytical optical dosimetry models for PDT treatment planning. The models will be tested at the photophysical level with tissue phantoms, and evaluated for applicability be retrospective comparison to on-going PDT trails.