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Coordinated Photoreceptor Engineering for Improved Biomass Production

协调光感受器工程以提高生物质生产

基本信息

批准号：
BB/V00056X/1
负责人：
John Christie
金额：
$ 62.85万
依托单位：
University of Glasgow
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2021
资助国家：
英国
起止时间：
2021 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FV00056X%2F1
关键词：
Coordinated Photoreceptor Engineering Improved Biomass

项目摘要

A key challenge for plant biologists has been to increase plant yield by altering photosynthetic productivity to secure food, energy and environmental sustainability. Phototropin receptor kinases (phot1 and phot2) play an important role in this regard by coordinating multiple light-capturing processes. These include phototropism, chloroplast movements, leaf positioning, leaf expansion and stomatal opening all of which influence a plant's photosynthetic competence by improving the efficiency of light capture, reducing photodamage, and regulating gas exchange between leaves and the atmosphere. However, little effort has been made to target these pathways for improving biomass production. We have recently shown that targeted engineering can be used successfully to modify the light-sensing properties of the phototropins and increase their sensitivity to light. Plants with these engineered photoreceptors exhibit more rapid and robust chloroplast movement responses and improved leaf positioning and expansion, leading to over 2-fold increases biomass accumulation under light-limiting conditions. These findings demonstrate the feasibility of engineering photoreceptors for improved plant growth and offer additional opportunities for enhancing plant yield through increases in photosynthetic competence. This proposal capitalises on these findings and will use a combinatorial protein engineering strategy to better synchronise the actions of the phototropins more effectively over a wider range of light conditions without causing detrimental high-light stress. Design principles for better synchronisation between phototropin receptors will be established first in Arabidopsis and extended to crops using barley as a model. Thus, a major outcome of this interlinked effort will be to establish a structural and functional blueprint for constructing engineered photoreceptors that will provide a non-transgenic, genome editing-based approach to coordinate stepwise enhancements in photosynthetic performance with an aim to increasing crop yield. This work will concurrently generate new photoreceptor components with tailored photochemical properties that will have utility in generating synthetic protein-based switches designed to regulate target cellular activities by light. As our knowledge of phototropin receptor signalling is still far complete, this proposal will also exploit the availability of a novel desensitised photoreceptor mutant background, identified from our phototropin engineering work, to identify new components of phototropin signalling through genetic suppressor screening. Together, the fundamental and applied aspects of this research will expand our understanding of phototropin receptor function and harness the ability to fine tune their action as a bona fide means to modulate yield for agronomic gain.

植物生物学家面临的一个关键挑战是通过改变光合作用生产力来提高植物产量，以确保食物、能源和环境的可持续性。趋光性激素受体激酶(Phot1和Pho2)通过协调多个光捕捉过程在这方面发挥重要作用。这些影响包括向光性、叶绿体运动、叶片定位、叶片扩张和气孔开放，所有这些都通过提高光捕获效率、减少光损伤和调节叶片与大气之间的气体交换来影响植物的光合作用能力。然而，针对这些提高生物质生产的途径所做的努力很少。我们最近已经证明，靶向工程可以成功地用于修饰趋光素的光敏特性，并增加它们对光的敏感性。带有这些光感受器的植物表现出更快速和更强大的叶绿体运动反应，并改善了叶片的定位和展开，导致在限光条件下生物量积累增加了2倍以上。这些发现证明了设计光感受器来改善植物生长的可行性，并为通过提高光合作用能力来提高植物产量提供了额外的机会。这项建议充分利用了这些发现，并将使用组合蛋白质工程策略，在更广泛的光照条件下更有效地同步趋光素的行动，而不会造成有害的强光胁迫。更好地同步促光激素受体之间的设计原则将首先在拟南芥中建立，并推广到以大麦为模型的作物中。因此，这一相互关联的努力的一个主要成果将是建立一个构建工程光感受器的结构和功能蓝图，该蓝图将提供一种非转基因的、基于基因组编辑的方法来协调光合作用表现的逐步增强，以提高作物产量。这项工作将同时产生具有定制的光化学性质的新的光感受器组件，这些组件将用于产生基于合成蛋白质的开关，该开关旨在通过光调节目标细胞的活动。由于我们对趋光性激素受体信号转导的了解仍然很完整，该建议还将利用我们的促性腺激素工程工作中鉴定的新型不敏感的感光细胞突变背景，通过基因抑制基因的筛选来识别趋光性激素信号的新成分。总之，这项研究的基础和应用方面将扩大我们对趋光素受体功能的理解，并利用微调其作用的能力作为一种真正的手段来调节产量以获得农艺收益。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The fluorescent protein iLOV as a reporter for screening of high-yield production of antimicrobial peptides in Pichia pastoris.

DOI：
10.1111/1751-7915.14034
发表时间：
2022-07
期刊：
Microbial biotechnology
影响因子：
5.7
作者：
通讯作者：

Regulation of plant phototropic growth by NPH3/RPT2-like substrate phosphorylation and 14-3-3 binding.

DOI：
10.1038/s41467-021-26333-5
发表时间：
2021-10-21
期刊：
Nature communications
影响因子：
16.6
作者：
Sullivan S;Waksman T;Paliogianni D;Henderson L;Lütkemeyer M;Suetsugu N;Christie JM
通讯作者：
Christie JM

Regulation of Plant Phototropic Growth by NPH3/RPT2-like Substrate Phosphorylation and 14-3-3 Binding

NPH3/RPT2 样底物磷酸化和 14-3-3 结合对植物向光性生长的调节