MOLECULAR MECHANISMS OF DIFFERENTIATION
分化的分子机制
基本信息
- 批准号:3273231
- 负责人:
- 金额:$ 39.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1978
- 资助国家:美国
- 起止时间:1978-07-01 至 1987-03-31
- 项目状态:已结题
- 来源:
- 关键词:Saccharomyces cell differentiation cell fusion cell type fungal genetics gene complementation gene dosage gene expression gene mutation genetic manipulation genetic mapping genetic markers genetic recombination genetic regulation genetic strain micromanipulator molecular biology pheromone spores temperature sensitive mutant
项目摘要
In Eucaryotes, normal development, as opposed to neoplastic growth, entails the
orderly differentiation of cells within a narrowly defined temporal and spatial
framework. Homothallism, inherited switch of the mating type locus in bakers
yeast, Saccharomyces cerevisiae, is exploited as a model system to study the
mechanisms involved in differentiation of cell types. Yeast cell types are
controlled by two allelic forms of the mating type locus (MAT) a and alpha.
Homothallic strains can change these cell types by genetic alterations at MAT in
a non-random fashion as often as every cell generation. We have been working on
the mechanism of mating type switching. The results obtained allow us to
conclude that yeast contains silent mating type information at loci HMa and HM
alpha which are loosely linked to MAT. We have shown that during the switching
process a replica of the silent information replaces the information existing at
the mating type locus. We have also obtained mutants which allow the expression
of the silent information in situ at HMa and HM alpha. Also, we are isolating
and characterizing mutants of the MAT locus to investigate as to how this locus
controls the cell type.
在真核生物中,正常发育,而不是肿瘤生长,需要
细胞在有限的时间和空间内有序分化
框架. 同宗配合,面包师交配型位点的遗传开关
酵母,酿酒酵母,被利用作为一个模型系统来研究
参与细胞类型分化的机制。 酵母细胞类型
由交配型基因座(MAT)a和α的两种等位基因形式控制。
同宗配合系菌株可以通过MAT的遗传改变改变这些细胞类型,
每一代细胞都有一个非随机的方式 我们一直努力设法
配对式开关的机制。 获得的结果使我们能够
结论是酵母在HMa和HM位点含有沉默的交配型信息
与MAT松散相连的alpha。 我们已经证明,在切换过程中,
处理静默信息的副本替换存在于
交配型基因座 我们还获得了允许表达
在HMa和HM alpha上的沉默信息。 另外,我们正在隔离
并表征MAT基因座的突变体,以研究该基因座如何
控制单元格类型。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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AMAR J KLAR其他文献
AMAR J KLAR的其他文献
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{{ truncateString('AMAR J KLAR', 18)}}的其他基金
1999 GORDON RESEARCH CONFERENCE ON EPIGENETIC EFFECTS
1999 年戈登表观遗传效应研究会议
- 批准号:
6043619 - 财政年份:1999
- 资助金额:
$ 39.29万 - 项目类别:
COLD SPRING HARBOR ADVANCED BACTERIAL GENETICS COURSE
冷泉港高级细菌遗传学课程
- 批准号:
3434862 - 财政年份:1980
- 资助金额:
$ 39.29万 - 项目类别:
COLD SPRING HARBOR ADVANCED BACTERIAL GENETICS COURSE
冷泉港高级细菌遗传学课程
- 批准号:
3434863 - 财政年份:1980
- 资助金额:
$ 39.29万 - 项目类别:
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