MOLECULAR MECHANISMS OF DIFFERENTIATION

在真核生物中，正常发育，而不是肿瘤生长，需要 在狭义的时间和空间内细胞的有序分化 框架。面包师交配型基因座的同型遗传开关 以酿酒酵母为模型系统，研究了酵母菌对微生物生长的影响。 参与细胞类型分化的机制。酵母细胞类型有 由交配型基因座(MAT)a和α的两种等位基因形式控制。 同系菌株可以通过基因改变改变这些细胞类型。 一种非随机的方式，就像每代细胞一样。我们一直在努力 交配型转换的机制。所获得的结果使我们能够 结论：酵母在HMA和HM基因座上含有沉默交配型信息 阿尔法，松散地连接到垫子上。我们已经证明，在转换过程中 处理静默信息的复制品将替换位于 交配型基因座。我们还获得了突变体，它允许表达 在HMA和HM Alpha的现场无声信息。另外，我们正在隔离 并对MAT基因座的突变体进行表征，以研究该基因座如何 控制单元格类型。

In Eucaryotes, normal development, as opposed to neoplastic growth, entails the orderly differentiation of cells within a narrowly defined temporal and spatial framework. Homothallism, inherited switch of the mating type locus in bakers yeast, Saccharomyces cerevisiae, is exploited as a model system to study the mechanisms involved in differentiation of cell types. Yeast cell types are controlled by two allelic forms of the mating type locus (MAT) a and alpha. Homothallic strains can change these cell types by genetic alterations at MAT in a non-random fashion as often as every cell generation. We have been working on the mechanism of mating type switching. The results obtained allow us to conclude that yeast contains silent mating type information at loci HMa and HM alpha which are loosely linked to MAT. We have shown that during the switching process a replica of the silent information replaces the information existing at the mating type locus. We have also obtained mutants which allow the expression of the silent information in situ at HMa and HM alpha. Also, we are isolating and characterizing mutants of the MAT locus to investigate as to how this locus controls the cell type.