INTERSTITIAL TISSUE AND ONCOTIC PRESSURE IN TRAUMA

创伤中的间质组织和胶体渗透压

基本信息

批准号：
3272638
负责人：
RICHARD M PETERS
金额：
$ 17.15万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
1977
资助国家：
美国
起止时间：
1977-07-01 至 1987-06-30
项目状态：
已结题

项目摘要

The specific aim is to study the effects of burn, accidental, and surgical trauma on the Starling pressures (capillary-blood oncotic pressure Pic, interstitial-fluid oncotic pressure Pit, and interstitial fluid pressure Pt). Emphasis will be placed on comparing the efficacy of various resuscitation fluids to maintain fluid homeostasis across the capillary and cellular membranes. The role of intracellular swelling in skeletal muscle ischemia will also be investigated. These objectives will be approached by newly developed techniques for measuring Starling pressures in various tissues of trauma patients and experimental animals. Interstitial fluid pressure Pt will be continuously monitored by the wick catheter technique. Interstitital-fluid oncotic pressure Pit will be measured directly in 1-5 Mul samples of fluid collected by empty wick catheters. Oncotic pressure will be determined in blooc samples collected at various periods in each experimental protocol. Needle biopsies of muscle from the experimental animals and some trauma patients will be used to study fluid shifts across cellular membranes and tissue compliance using morphometric techniques. Human investigations will be made of fluid resuscitation for patients undergoing aortic reconstruction, cardiopulmonary bypass, and following burn injury, long bone fractures, the postural effects on Starling pressures in thorax and ankle, brawny edema following cast removal. In most of these protocols, animal studies will parallel our clinical investigations. Our patient studies will define changes in clinical situations and will allow longitudinal studies and parallel animal experiments will fill in gaps in the clinical studies. Our long-term objective is to identify and quantitate water, protein and electrolyte shifts across capillary and cellular membranes after trauma and subsequent fluid resuscitation to formulate the most effective means of fluid resuscitation for each type of trauma.

具体目的是研究烧伤，偶然和手术的影响 Starling压力上的创伤（毛细血管血肿瘤压力图片，间质流体肿瘤压力坑和间质流体压力 pt）。重点将放在比较各种功效上复苏液以维持整个毛细管的液体稳态和细胞膜。细胞内肿胀在骨骼肌中的作用还将研究缺血。这些目标将由新开发的技术，用于测量各种八度压力创伤患者和实验动物的组织。间质流体压力PT将通过WICK导管技术不断监测。室内液体肿瘤压力坑将直接在1-5中测量由空芯导管收集的流体样品。肿瘤压力将在每个时期收集的BLOOC样品中确定实验方案。实验性的肌肉的针头活检动物和一些创伤患者将用于研究跨越液体的转移使用形态计量技术的细胞膜和组织依从性。人类研究将对患者进行液体复苏进行主动脉重建，心肺旁路和关注烧伤损伤，长骨骨折，对Starling的姿势作用胸腔和踝关节的压力，脱落后的野蛮水肿。在这些方案中的大多数，动物研究将平行我们的临床调查。我们的患者研究将定义临床的变化情况并将允许纵向研究和平行动物实验将填补临床研究中的空白。我们的长期目的是识别和定量水，蛋白质和电解质创伤后跨毛细管和细胞膜的转移流体复苏以制定最有效的流体手段每种类型的创伤的复苏。