THERMAL INJURY, COMPLEMENT AND LEUKOCYTE DYSFUNCTION

我们最近对大鼠皮肤热损伤的研究表明， 开始全身激活补体，获得吞噬细胞 继发性肺损伤的缺陷和发展。肺 并发症与中性粒细胞在血管内的激活有关， 白细胞聚集物在肺内的滞留与肺间质纤维化的发生 氧自由基(羟基自由基)致血管内皮损伤 由白细胞形成。我们现在计划研究两个局部影响 热损伤以及一些全身并发症(吞噬细胞 局部热引起的细胞缺陷和血管内溶血) 皮肤损伤。拟议的研究将侧重于调解人制度。 负责局部对热损伤的炎症反应， 热损伤的实质细胞和间充质细胞的激活能力 补充性制度、调解人对后天缺陷的责任 烫伤后吞噬细胞的初步研究 有证据表明血管内溶血与热有关 损伤可能是血管内激活的综合结果 补体以及血液中性粒细胞产生的氧自由基。这个 流式细胞仪定量分析技术的研究进展 从小体积(0.1毫升)的全血中分离出未分离的白细胞 老鼠血将极大地促进这些研究。

Our recent studies of skin thermal injury in rats have demonstrated the onset of systemic activation of complement, acquisition of phagocytic cell defects and development of secondary lung injury. The pulmonary complication has been related to intravascular activation of neutrophils, entrapment of leukoaggregates in lungs and the development of pulmonary vascular endothelial injury due to oxygen radical (hydroxyl radical) formation by leukocytes. We now plan to examine both local effects of thermal injury as well as some of the systemic complications (phagocytic cell defects and intravascular hemolysis) arising from localized thermal injury of skin. The proposed studies will focus on mediator systems responsible for the local inflammatory response to thermal injury, the ability of thermally injured parenchymal and mesenchymal cells to activate the complement system, mediators responsible for the acquired defects of phagocytic cells following thermal injury, and the pursuit of preliminary evidence suggesting that intravascular hemolysis associated with thermal injury is probably the combined result of intravascular activation of complement as well as oxygen radical generation by blood neutrophils. The development of flow cytometric techniques to analyze in a quantitative manner unfractionated blood leukocytes from small volumes (0.1 ml) of whole rat blood will greatly facilitate these studies.